Enterokinase from porcine intestine
SIGMA/E0632 - ≥0.5 units/mg solid
Synonym: Enteropeptidase
CAS Number: 9014-74-8
EC Number: 232-761-1
MDL Number: MFCD00131020
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: E0632-1.5KU
| form | salt-free, lyophilized powder |
| mol wt | 150 kDa |
| Quality Level | 200  |
| specific activity | ≥0.5 units/mg solid |
| storage temp. | −20°C |
| Application: | Enterokinase from porcine intestine has been used in a study to report a new experimental model of the anomalous pancreatico-biliary junction. Enterokinase from porcine intestine has also been used in a study to investigate the insulinotropic region of the gastric inhibitory polypeptide. |
| Application: | The enzyme from Sigma has been used for the activation of trypsinogen in order to measure the activity of trypsin in hog pancreas. The study showed that antimicrobial treatment reduces intestinal microflora and improves protein digestive capacity without changes in villous structure of weanling pigs. |
| Biochem/physiol Actions: | Enterokinase is a membrane bound serine protease that specifically and rapidly converts trypsinogen to trypsin, thereby, triggering the conversion of other zymogens to active enzymes. It has a molecular mass of approximately 150 kDa. The enzyme is a heterodimer consisting of 35-47 kDa subunits. The light and the heavy chains are linked by two disulfide bridges. It is a glycoprotein containing 35% carbohydrate. The polypeptide chain of trypsinogen is hydrolyzed only after an -(Asp)4-Lys- sequence. The enzyme is inhibited by soybean trypsin inhibitor. Enterokinase is typically used in protein modification and amino acid sequence determination. |
| Other Notes: | One unit will produce 1.0 nanomole of trypsin from trypsinogen per min at pH 5.6 at 25 °C. |
| Packaging: | 1500 units in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥0.5 units/mg solid |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.21.9 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |