Endothelial cell growth supplement from bovine pituitary
SIGMA/E0760 - ECGS, BioReagent, suitable for cell culture
Synonym: ECGS; Endothelial Mitogen
Product Type: Chemical
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| biological source | bovine pituitary glands |
| color | brown to tan |
| form | powder |
| impurities | ≤20 EU/vial (LAL test) |
| packaging | pkg of 15 mg |
| potency | 2-300 μg/mL ED50/EC50 |
| product line | BioReagent |
| quality | endotoxin tested |
| Quality Level | 200  |
| solubility | water: soluble to hazy, red to red-brown (1 vial reconstituted with 1 ml 0.85% NaCl) |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Endothelial cell growth supplement from bovine pituitary has been used: • as a component in Roswell park memorial institute (RPMI) 1640 medium for endothelial cell culture • as a supplement in medium 199 for endothelial cell culture • as a supplement in Dulbecco′s modified eagle medium (DMEM):Ham′s F-12 • as a supplement in Iscove′s modified Dulbecco′s medium (IMDM) for human umbilical vein endothelial cells (HUVEC) culture |
| Biochem/physiol Actions: | Endothelial cell growth supplement (ECGS) has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production. |
| General description: | Endothelial cell growth supplement (ECGS) is an extract of bovine pituitary glands containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth supplement is prepared using a modification of a published method. It is lyophilized from a sterile solution containing NaCl and streptomycin sulfate. |
| Symbol |  GHS08 |
| Signal word | Warning |
| Hazard statements | H361fd |
| Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
| Hazard Codes | Xn |
| Risk Statements | 63 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |