Benzonase® Nuclease
SIGMA/E1014 - ≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
Synonym: Endonuclease from Serratia marcescens
CAS Number: 9025-65-4
MDL Number: MFCD00131010
Product Type: Chemical
Hydrolyzes double and single-stranded DNA and RNA yielding 5′-phosphomononucleotide and 5′-phosphooligonucleotide end-products.
| application(s) | research use |
| assay | ≥90% (SDS-PAGE) |
| biological source | Serratia marcescens |
| concentration | ≥250 units/μL |
| foreign activity | protease, essentially free |
| form | buffered aqueous glycerol solution |
| mol wt | 30 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| Application: | Benzonase® Nuclease has been used: as a component in ice-cold lysis buffer C to digest DNA and RNA to facilitate the complete release of all nuclear proteins in the immunoprecipitation step to release protein complexes from the nucleoplasm and chromatinas a supplement in RIPA to fractionate SHSY5Y cells for immunoprecipitation to remove residual nucleic acids from the aortic roots in decellularization method |
| Application: | Used for the removal of nucleic acid from protein samples. |
| Biochem/physiol Actions: | Benzonase® Nuclease can completely digest nucleic acids into 5′-monophosphate terminated oligonucleotides of 3 to 5 bases in length, making it the ideal tool for removing nucleic acids from recombinant proteins and for applications that require complete digestion of nucleic acids. In addition to reducing viscosity in protein extracts and preventing cell clumping, pretreatment of protein samples with Benzonase® nuclease can significantly improve their resolution on 2D gel electrophoresis by eliminating any bound nucleic acids. This versatile enzyme can digest both native or heat-denatured DNA and RNA, with its optimum pH for enzyme activity found to be 8.0-9.2. Benzonase® nuclease is effective at removing host DNA from microbiome samples. In many cases, microbiome samples (such as saliva or skin) will have a high percentage of host DNA that interferes with downstream results. Our experts show that the reduction of host DNA lowers the cost of sequencing while increasing and improving the data. Experimental data is shown in the technical article - Benzonase® Nuclease for Microbiome Workflows |
| Biochem/physiol Actions: | Digests native or heat-denatured DNA and RNA. |
| Features and Benefits: | • Host DNA depletion in microbiome samples. • Effective nucleic acid digestion in a variety of workflows. • Viscosity reduction during protein extraction. |
| General description: | Benzonase® nuclease is a highly efficient and genetically engineered endonuclease that originates from Serratia marcescens. This dimeric protein with two essential disulfide bonds is capable of attacking and degrading all forms of DNA and RNA (single-stranded, double-stranded, linear, and circular) under a wide range of operating conditions. Benzonase® nuclease is capable of removing nucleic acids and enhancing the purity and quality of protein samples. |
| Legal Information: | Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | Benzonase® Nuclease is supplied by Merck KGaA, Darmstadt, Germany and/or its affiliates. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Other Notes: | One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C (reaction volume 2.625 ml). |
| Physical form: | Solution in 50% glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl2, and 20 mM NaCl. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.30.2 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |