Anti-ERGIC-53/p58 antibody produced in rabbit
SIGMA/E1031 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-ERGIC53; Anti-F5F8D; Anti-FMFD1; Anti-LMAN1; Anti-MCFD1; Anti-MR60; Anti-gp58
MDL Number: MFCD07370310
Product Type: Chemical
Upper panels. NIH 3T3 cells transiently expressing a β-(1-3)galactosyltransferase-YFP fusion protein (GalT) were stained with Rabbit Anti-ERGIC53 primary (Cat. No. E1031) and Goat Anti-Rabbit IgG-Cy5 conjugate secondary antibodies. Samples were analyzed on a Zeiss LSM confocal microscope. Note that although in the same region of the cell, ERGIC53 shows a different pattern and little overlap with the medial Golgi marker GalT.
Lower panels. Double-label immunofluorescence of NIH 3T3 cells stained with Rabbit Anti-ERGIC53 as in the upper panels and with Mouse Anti-β-COP primary (Cat. No. G6160) and Goat Anti-Mouse IgG-FITC conjugated secondary antibodies. Note that β-COP concentrates mainly in the Golgi with some staining of the ER to Golgi intermediate compartment (ERGIC), therefore overlapping partially with ERGIC53. Some β-COP also stains peripheral vesicles.
The pictures are courtesy of E. Avezov and G.Z. Lederkremer, Dept. of Cell Research and Immunology, Tel-Aviv University, Tel-Aviv, Israel.
Lower panels. Double-label immunofluorescence of NIH 3T3 cells stained with Rabbit Anti-ERGIC53 as in the upper panels and with Mouse Anti-β-COP primary (Cat. No. G6160) and Goat Anti-Mouse IgG-FITC conjugated secondary antibodies. Note that β-COP concentrates mainly in the Golgi with some staining of the ER to Golgi intermediate compartment (ERGIC), therefore overlapping partially with ERGIC53. Some β-COP also stains peripheral vesicles.
The pictures are courtesy of E. Avezov and G.Z. Lederkremer, Dept. of Cell Research and Immunology, Tel-Aviv University, Tel-Aviv, Israel.
Immunofluorescence Hepg2 human hepatocellular carcinoma cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 μg/mL Anti-ERGIC-53/p58 antibody produced in Rabbit (Cat. No. E1031). The antibody was developed using Goat Anti-Rabbit IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunofluorescence NRK normal Rat kidney cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 μg/mL Anti-ERGIC-53/p58 antibody produced in Rabbit (Cat. No. E1031). The antibody was developed using Goat Anti-Rabbit IgG, Cy3™.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~58 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 5-10 μg/mL using human HepG2 or rat NRK cells |
| western blot (chemiluminescent): 0.5-1.0 μg/mL using whole cell extract of mouse NIH3T3 cells | |
| UniProt accession no. | P49257  |
| Application: | Anti-ERGIC-53/ (p58) antibody can be used in immunoblotting and immunofluorescence. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | ERGIC (ER-Golgi intermediate compartment) is related to type I membrane marker protein ERGIC-53 and rat homolog is referred as (p58) . ERGIC is a dynamic membrane system made up of tubulo vesicular clusters near to ER exit site which facilitates transport of protein from ER to Golgi. Anti-ERGIC-53/ (p58) antibody can be used as a primary antibody (diluted 1: 125) in fluorescence microscopy. It may also be used for western blotting. Rabbit anti-ERGIC-53/ (p58) antibodies react specifically with ERGIC-53/ (p58) of rat, mouse and human. |
| Immunogen: | synthetic peptide corresponding to amino acids 158-170 of rat p58 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse and human. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |