Anti-Ezrin antibody produced in rabbit
SIGMA/E1281 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-80K; Anti-Cytovillin; Anti-p81
MDL Number: MFCD00211933
Product Type: Chemical
Immunofluorescence A431 cells were fixed and permeabilized with 4% Paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 μg/mL Rabbit Anti-Ezrin (Cat. No. E1281). The antibody was developed using Goat Anti-Rabbit IgG, Atto 488 conjugate (Cat. No. 18772). Cells were counterstained with DAPI (blue) to stain nuclei.
Western Blotting Whole extract of NRK cells were separated on SDS-PAGE and probed with Anti-Ezrin antibody produced in Rabbit (Cat. No. E1281). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody 3. 1 μg/mL antibody + inhibitor 4. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~80 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 20-40 μg/mL |
| immunoprecipitation (IP): 0.5-1 μg using RIPA extract of human A431 cells | |
| indirect immunofluorescence: 10-20 μg/mL | |
| western blot (chemiluminescent): 0.5-1 μg/mL using whole extract of rat NRK cells | |
| UniProt accession no. | P15311  |
| Application: | Anti-Ezrin antibody produced in rabbit has been used in: • immunolocalization studies • immunostaining • immunofluorescence and immunohistochemistry • immunoblot |
| Biochem/physiol Actions: | Cultured neuronal cells do express ezrin to some extent, and whole brain tissue expresses low levels of ezrin. Ezrin expression is associated with malignancy in astrocytoma, osteosarcoma, mammary and pancreatic adenocarcinomas, and in cutaneous and uveal melanomas. |
| Biochem/physiol Actions: | Ezrin promotes cell adhesion. The NH2-terminal domain negatively regulates membrane spreading and elongation properties of ezrin COOH-terminal domain. The members of ERM family are usually involved in cross-linking of plasma membrane and actin filaments. ERM proteins are involved in cell adhesion, cell shape determination, migration, and the organization of cell surface structures. They are also involved in signal transduction and apoptosis. Ezrin has been implicated in tumor progression and metastatic spread. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Ezrin is a 80kDa membrane-associated protein that belongs to ERM (Ezrin/Radixin/Moesin) family. Ezrin is found predominantly in polarized epithelial cells such as intestinal epithelial cells, gastric parietal cells, epithelial cells of the kidney proximal tubule, terminal bronchiole of the lung, and in mesothelia. |
| Immunogen: | synthetic peptide corresponding to amino acids 479-498 of human ezrin with N-terminal added cysteine, conjugated to KLH. The corresponding sequence in rat and mouse differs by three amino acids. |
| Physical form: | Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |