β-Endothelial Cell Growth Factor human
SIGMA/E1388 - β-ECGF, recombinant, expressed in E. coli, suitable for cell culture
Synonym: ECGS; β-ECGF
CAS Number: 105843-51-4
MDL Number: MFCD00131012
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| assay | ≥97% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | ≤1.0 EU/μg protein Endotoxin level (LAL test) |
| mol wt | predicted mol wt ~17 kDa |
| packaging | pkg of 25 μg |
| potency | 0.03-0.3 ng/mL ED50/EC50 |
| quality | endotoxin tested |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage condition | avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.) |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P49765  |
| Analysis Note: | The proliferative activity is tested in culture by using quiescent NR6R-3T3 fibroblasts. |
| Application: | β-Endothelial Cell Growth Factor human has been used: • in cell death assay • in endothelial cell culture • in human umbilical vascular endothelial cell (HUVEC) culture |
| Biochem/physiol Actions: | β-Endothelial cell growth factor (β-ECGF) is a potent endothelial cell mitogen and its mitogenic activity is potentiated by heparin. β-ECGF stimulates the growth of quiescent HUVEC cells in culture. β-ECGF is associated with lipid and glucose metabolism. This growth factor induces neovascularization in disease conditions. Upregulation of β-ECGF is observed in diabetic retinopathy, and polycystic ovary syndrome. It is also associated with tumor proliferation and invasion. β-ECGF increases fatty acid transport proteins expression to improve endothelial mediated lipid transpor. |
| General description: | The β-endothelial cell growth factor (b-ECGF) gene encodes an angiogenic factor, and is mapped to human chromosome 11q13.1. Recombinant, human b-endothelial cell growth factor (b-ECGF) is a 14 amino acid N-terminal extension of acidic fibroblast growth factor. It is expressed in Escherichia coli. The 154 amino acid recombinant protein, generated after removal of the initiation methionine, has a predicted molecular mass of about 17 kDa. |
| Other Notes: | 14 amino acid, N-terminal extended form of human FGF-acidic. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution in 10 mM MOPS, 50 mM ammonium sulfate, 0.2 mM DTT, and 0.2 mM EDTA, pH 7.2, containing 50 μg bovine serum albumin per 1 μg cytokine. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |