Proline Specific Endopeptidase from Flavobacterium sp.
SIGMA/E1411 - lyophilized powder, ≥5.0 units/mg solid
Synonym: EndoProC; Endoprolylpeptidase; Endoproteinase Pro-C; PEPase; Prolyl Endopeptidase
Product Type: Chemical
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| biological source | bacterial (Flavobacterium spp.) |
| form | lyophilized powder |
| mol wt | ~78 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| specific activity | ≥5.0 units/mg solid |
| storage temp. | −20°C |
| Application: | Proline specific endopeptidase is useful for the determination of amino acid sequences of peptides and proteins containing proline residues. Proline specific endopeptidases may be useful in cancer, neurology and diabetes research . Prolyl endopeptidase (PEP) may be used as a drug target for neuropsychiatric diseases such as stress disorder, depression, and schizophrenia . |
| Biochem/physiol Actions: | An endopeptidase is a proteolytic peptidase that breaks peptide bonds of nonterminal amino acids. Product E1411 is specific for proline (Pro). The Prolyl endopeptidase (PEP) mechanism is thought to be an induced fit mechanism, where the native enzyme exists in a conformationally flexible opened state but is changed to a closed state upon substrate binding . |
| Other Notes: | One unit causes the formation of one micromole of p-nitroaniline per minute under specific pH and temperature conditions. |
| Packaging: | 50 units in glass bottle |
| Physical form: | White amorphous lyophilized powder |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥5.0 units/mg solid |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |