Ethidium bromide solution
SIGMA/E1510 - BioReagent, Molecular Biology, 10 mg/mL in H2O
Synonym: 3,8-
CAS Number: 1239-45-8
Empirical Formula (Hill Notation): C21H20BrN3
Molecular Weight: 394.31
MDL Number: MFCD00011724
Linear Formula: C21H20BrN3
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: E1510-10ML
| concentration | 10 mg/mL in H2O |
| grade | Molecular Biology |
| InChI | 1S/C21H19N3.BrH/c1-2-24-2 |
| InChI key | ZMMJGEGLRURXTF-UHFFFAOYSA |
| product line | BioReagent |
| Quality Level | 200  |
| SMILES string | [Br-].CC[n+]1c(-c2ccccc2) |
| suitability | suitable for gel electrophoresis |
| technique(s) | electrophoresis: suitable |
| Application: | Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. |
| Application: | Ethidium bromide solution has been used: • as a stain to visualize U937 cells to assess cell viability • to detect polymerase chain reaction products • in agarose gel electrophoresis based gel retardation assay |
| Biochem/physiol Actions: | Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Packaging: | 10 mL in glass bottle |
| Preparation Note: | For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis. |
| Symbol |   GHS06,GHS08 |
| Signal word | Danger |
| Hazard statements | H331 - H341 |
| Precautionary statements | P202 - P261 - P271 - P280 - P304 + P340 + P311 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 23-68 |
| Safety Statements | 36/37-45 |
| RIDADR | UN 2810 6.1 / PGIII |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41105322 |