Anti-ERK5 (big-MAPK, BMK1) antibody produced in rabbit
SIGMA/E1523 - IgG fraction of antiserum, buffered aqueous solution
MDL Number: MFCD01633332
Product Type: Chemical
Western Blotting Whole extract of NIH-3T3 cells were separated on SDS-PAGE and probed with Anti-ERK5 (big-MAPK, BMK1) antibody produced in Rabbit (Cat. No. E1523). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:8,000 antibody 2. 1:16,000 antibody 3. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 97 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | microarray: suitable |
| western blot: 1:8,000 using NIH 3T3 mouse fibroblasts cell extract | |
| UniProt accession no. | Q13164  |
| Application: | Anti-ERK5 (big-MAPK, BMK1) an0tibody produced in rabbit has been used in immunoblotting. |
| Biochem/physiol Actions: | Extracellular signal-regulated kinase 5 (ERK5) has a critical role in different cellular processes like differentiation, proliferation and survival. Anti-ERK5 (big-MAPK, BMK1) antibody can be used to study the differential tissue expression and intracellular localization of the MAP Kinase isoform in normal and neoplastic tissues. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Extracellular signal-regulated kinase 5 (ERK5), also referred to as big-MAP kinase 1 (BMK1), is a protein that belongs to mitogen-activated protein kinases family and is expressed mainly in skeletal muscle, kidney, heart and placenta. ERK5 contains a distinct 400-amino acid C-terminal and loop-12 domain, which includes two proline-rich regions. |
| Immunogen: | synthetic peptide corresponding to amino acids 789-802 of human ERK5. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |