Chondroitinase AC from Flavobacterium heparinum
SIGMA/E2039 - recombinant, expressed in E. coli, ≥200 units/mg protein, For Chondroitin Sulfate Analysis
Synonym: Chondroitin AC lyase
CAS Number: 9047-57-8
MDL Number: MFCD00130796
Product Type: Chemical
Chondroitinase A,B and C specificities
SDS-PAGE The purity of recombinant Chondroitinase AC (Cat. No. E2039) was determined by SDS-PAGE of reduced Chondroitinase AC and staining with Coomassie Blue.
| assay | ≥90% (SDS-PAGE) |
| conjugate | (Glucosaminoglycan) |
| form | lyophilized solid |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| specific activity | ≥200 units/mg protein |
| storage temp. | −20°C |
| Application: | Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate). |
| Application: | Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements. |
| Application: | Highly purified to remove interferring β-glucuronidase and protease activities for use in the hydrolysis of chondroitin sulfate pror to HPLC analysis. |
| Other Notes: | 1 unit is defined as the amount of enzyme that will liberate 1.0 μmole per minute of unsaturated disaccharides from chondroitin sulfate A at pH 6.7 at 37 °C as measured by the change in A232. The εμΜ for the reaction product Δ-Di-4S (chondroitin sulfates A and B) is 5.1 and 5.5 for Δ-Di-6S (chondroitin sulfate C). |
| Other Notes: | View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enz |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥90% (SDS-PAGE) |
| activity | specific activity: ≥200 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 4.2.2.5 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |