Evans Blue

SIGMA/E2129 - Dye content ≥75 %

Synonym: Direct Blue 53

CAS Number: 314-13-6
Empirical Formula (Hill Notation): C₃₄H₂₄N₆Na₄O₁₄S₄
Molecular Weight: 960.81
EC Number: 206-242-5
MDL Number: MFCD00004021
Linear Formula: C₃₄H₂₄N₆Na₄O₁₄S₄
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-E2129-10G	10 g	$70.50 1/EA	Add To Favorites
45-E2129-50G	50 g	$280.00 1/EA	Add To Favorites
45-E2129-100G	100 g	$462.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: E2129-100G

Properties
Descriptions
Safety Info.
Basic Data

application(s)	diagnostic assay manufacturing hematology histology
composition	Dye content, ≥75%
form	powder
InChI	1S/C34H28N6O14S4.4Na/c1-15-11-17(3-7-21(15)37-39-23-9-5-19-25(55(43,44)45)13-27(57(49,50)51)31(35)29(19)33(23)41)18-4-8-22(16(2)12-18)38-40-24-10-6-20-26(56(46,47)48)14-28(58(52,53)54)32(36)30(20)34(24)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-37+,40-38+;;;;
InChI key	ATNOAWAQFYGAOY-GPTZEZBUSA-J
Quality Level	200
SMILES string	[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2ccc3c(cc(c(N)c3c2O)S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5ccc6c(cc(c(N)c6c5O)S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
storage temp.	room temp
technique(s)	titration: suitable

Application:	Evans blue is a well-known vital stain with applications in histology and fluorescent microscopy. Evans blue has been used to check cell viability and study blood-brain barrier (BBB) permeability. Additional applications include the demonstration of specific pituitary cell types, selective staining of neutrophil plasma membranes, and fluorescence microscopy as a quenching agent to suppress unwanted autofluorescence.
Biochem/physiol Actions:	Evans blue is a non-permeating dye. In presence of plasma membrane damage, the dye enters in the cytoplasm and nucleus, thereby staining them blue. Evans blue is used for checking cell viability. It is also used to study BBB (blood-brain barrier) permeability. The dye binds to albumin and hence indicates BBB breakdown to protein.
General description:	Evans blue (EB) C.I. 23860 is a synthetic bis-azo dye also known as T-1824 and Direct Blue 53. EB dye with higher water solubility has a strong affinity towards serum albumin, making it a high molecular weight protein tracer.
Packaging:	10, 50, 100 g in glass bottle

Symbol	GHS08
Signal word	Danger
Hazard statements	H350
Precautionary statements	P201 - P202 - P280 - P308 + P313 - P405 - P501
Hazard Codes	T
Risk Statements	45
Safety Statements	53-45
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	room temp
Colour Index Number	23860
UNSPSC	12171500

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