Anti-EGF Receptor antibody, Mouse monoclonal
SIGMA/E2156 - clone 225, purified from hybridoma cell culture
Synonym: Anti-EGFR; Anti-Epidermal Growth Factor Receptor
MDL Number: MFCD00162282
Product Type: Chemical
Immunoprecipitation Anti-EGF Receptor antibody, Mouse monoclonal, Clone: 225 (Cat. No. E2156), was used to immunoprecipitate EGF Receptor from A431 cells extract. Detection antibody: 1:2,000 Anti-Epidermal Growth Factor Receptor antibody, Mouse monoclonal, Clone: 29.1 (Cat. No. E2760), followed by 1:8,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917) Lanes 1. 8 μg/mL antibody 2. 4 μg/mL antibody 3. Negative Control - without first antibody 4. Negative Control - without extract
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | 225, monoclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 4-8 μg using cell lysate of A431 cells |
| UniProt accession no. | P00533  |
| Application: | Monoclonal Anti-EGF Receptor antibody has been used in antibody-nanoparticle conjugation. |
| Application: | Monoclonal Anti-EGF Receptor antibody is suitable for use in immunoprecipitation (4-8 μg using cell lysate of A431 cells). |
| Biochem/physiol Actions: | EGFR (epidermal growth factor receptors) protein tyrosine kinase is activated when EGF binds the extracellular binding domain. The first detectable response is the autophosphorylation of the C-terminal tyrosine followed by phosphorylation of other endogenous substrates. |
| Biochem/physiol Actions: | EGFR is a tyrosine kinase receptor that regulates cellular several functions such as growth, blood vessel formation, metastasis and invasion. Alterations in EGFR expression have been associated with a wide range of cancers. Thus, drugs that target EGFR signaling have important therapeutic applications in cancer . |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-EGFR (mouse IgG1 isotype) is derived from the hybridoma 225 produced by the fusion of mouse myeloma cells (NS-1-503 cells) and splenocytes from BALB/c mice immunized with partially purified EGF receptors from A-431 cells. The receptor for epidermal growth factor (EGF) is an integral cell membrane glycoprotein of 170 kDa, which spans the membranes of a wide range of normal and malignant epithelial cells. The EGF receptor has an intracellular domain that exhibits tyrosine kinase activity. |
| Immunogen: | partially purified EGF receptors from human A-431 cells. |
| Legal Information: | This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California. |
| Physical form: | 0.2 μm filtered solution in 0.01 M phosphate buffered saline, pH 7.4. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |