Endoglycosidase F3 from Elizabethkingia miricola
SIGMA/E2264 - recombinant, expressed in E. coli, 30 U/mg
Synonym: Elizabethkingia miricola; Endo-β-N-acetylglucosaminidase F3; Endoglycosidase F3 from Elizabethkingia (Chryseobacterium/
MDL Number: MFCD00146843
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| conjugate | (N-linked) |
| form | solution |
| mol wt | 32 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| specific activity | 30 U/mg |
| storage temp. | 2-8°C |
| Application: | Endoglycosidase F3 from Elizabethkingia miricola has been used to analyze core fucosylation and tryptic digests of serum proteins. |
| Biochem/physiol Actions: | Cleaves asparagine-linked biantennary and triantennary complex, oligosaccharides depending on the state of core fucosylation and peptide linkage. |
| Biochem/physiol Actions: | Endoglycosidase F3 belongs to the glycoside hydrolase family 18 (GH18). It has hydrolytic activity. Endoglycosidase F3 glycosylates α-1,6-fucosylated GlcNAc derivative to give natural, core fucosylated complex-type N-glycopeptides. |
| Other Notes: | One unit will release N-linked oligosaccharides from 1 μmole of denatured porcine fibrinogen in 1 minute at 37 °C, pH 4.5. |
| Packaging: | Supplied with 5× Reaction Buffer, 250 mM sodium acetate, pH 4.5 |
| Physical form: | Aseptically filled solution in 20 mM Tris-HCl, pH 7.5 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 30 U/mg |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.96 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |