ExtrAvidin®-Agarose, high binding
SIGMA/E2513 - For purification of biotinylated macromolecules and complexes
Synonym: ExtrAvidin® beads; de-glycosylated Avidin
Product Type: Chemical
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| concentration | 50% |
| feature | For purification of biotinylated macromolecules and complexes |
| form | aqueous suspension |
| suspension | |
| matrix | 4% agarose (beaded) |
| matrix activation | cyanogen bromide |
| storage temp. | 2-8°C |
| Application: | ExtrAvidin®-Agarose provides high affinity with high specificity binding to Biotin (also known as vitamin B7) including biotinylated proteins and biotin-tagged fusion proteins. The product may be used in various immunological techniques, including Immunoprecipitation and Immunoaffinity purification. |
| Disclaimer: | DO NOT FREEZE |
| Disclaimer: | This product is for research use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices. |
| General description: | ExtrAvidin® is a unique de-glycosylated Avidin modified form of an affinity purified egg white avidin. The ExtrAvidin is conjugated to cyanogen bromide-activated agarose beads. ExtrAvidin® is a tetrameric protein containing four biotin binding sites. The Avidin-Biotin high affinity interaction (Kd=10-15M) is considered one of the strongest non-covalent interactions known in nature. The use of Avidin-Biotin complex for affinity purification was described in the first half of 1970 and since then it was successfully utilized in numerous studies and biotechnological applications.1-3 This was achieved due to the ability to chemically coupe the small Biotin molecule with different binders, without disturbing its function or structure, thus allowing unique interaction with a variety of Avidin carriers including: protein or DNA molecules, Avidin protein bound to a solid surface matrix, reporter molecules, probes or carriers,. Among the known Avidin-Biotin interaction based applications are purification, enrichment, detection, amplification and other research medical and industrial processes. In addition, the ExtrAvidin® high specificity binding to Biotin, together with the low background staining, grant it significant advantage compared to the non-modified Avidin or Streptavidin produced by Streptomyces avidinii. |
| Legal Information: | ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | ExtrAvidin®-Agarose is provided as suspension at a 1:1 ratio, in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative. |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106500 |