Anti-Epidermal Growth Factor antibody, Mouse monoclonal
SIGMA/E2520 - clone EGF-10, purified from hybridoma cell culture
Synonym: Anti-CEGF
MDL Number: MFCD00239669
Product Type: Chemical
Western Blotting Whole extract of EGF Human recombinant cells were separated on SDS-PAGE and probed with Monoclonal Anti-Epidermal Growth Factor antibody produced in Mouse, Clone: EGF-10 (Cat. No. E2520). The antibody was developed using 1:1,000 Anti-Mouse IgG (whole molecule)-Peroxidase antibody. Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody 3. Negative Control
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | mouse |
| clone | EGF-10, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 6 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: 0.1 μg/mL using recombinant human EGF |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using human tissue | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| neutralization: suitable | |
| radioimmunoassay: suitable | |
| western blot: suitable | |
| UniProt accession no. | P01133  |
| Application: | Monoclonal Anti-Epidermal Growth Factor antibody has been used in dot-blot, radioimmunoassay, enzyme linked immunosorbent assay (ELISA), immunohistology, immunoblotting, immunostaining. |
| Application: | Monoclonal Anti-Epidermal Growth Factor antibody produced in mouse was used to study rat immune cells by flow cytometry to detect the presence or absence of hormone and hormone-like molecules in response to EGF treatment. |
| Biochem/physiol Actions: | EGFR is a transmembrane glycoprotein that binds to the EGF family. It is a pivotal regulator of cell proliferation, growth, differentiation and DNA repair after radiation-induced damage. Mutations in the gene encoding EGFR are associated with a number of malignancies including lung cancer and colorectal cancer. |
| Biochem/physiol Actions: | Epidermal growth factor (EGF) is a potent growth-promoting factor for a variety of tissue cells in vivo and in vitro. EGF binding induces phosphatidylinositide hydrolysis and generation of diacylglycerol (DAG). Cellular metabolic effects of EGF include stimulation of ion fluxes, glucose transport, glycolysis and synthesis of DNA, RNA and proteins. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells. The biological role of EGF includes the inhibition of gastric acid secretion, support of growth and differentiation during fetal development, neuromodulation in the central nervous system and stimulation of epidermal growth and keratinization. |
| Biochem/physiol Actions: | Specifically reacts with both native and recombinant human EGF. The antibody does not cross-react with mouse EGF. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Epidermal Growth Factor (EGF) (mouse IgG1 isotype) is derived from the EGF-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Epidermal growth factor (EGF) is a single chain polypeptide hormone (6 kDa). EGF protein exists largely in a β-sheet conformation, with a C-terminal hairpin that may be involved in receptor binding. Human EGF is found in body fluids such as urine, milk, saliva, sweat, and seminal fluid and has its highest concentration in α-granules of blood platelets. |
| Immunogen: | recombinant human EGF |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |