ExtrAvidin® –Alkaline Phosphatase
SIGMA/E2636 - buffered aqueous solution
Synonym: Alkaline Phosphatase Reagent; ExtrAvidin Alkaline Phosphatase
MDL Number: MFCD00163600
Product Type: Chemical
ELISA assay utilizing a biotinylated antibody and ExtrAvidin®−Alkaline Phosphatase detection.
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of human appendix stained with Monoclonal Anti-Actin, α-Smooth Muscle (Cat. No. A2547) followed by Biotin (Cat. No. B7151) and by 1:100 ExtrAvidin®-Alkaline Phosphatase (Cat. No. E2636).
ELISA 1 μg/mL Anti-Mouse IgG (whole molecule)-Biotin antibody produced in Rabbit (Cat. No. B8520) followed by serially diluted ExtrAvidin®-Alkaline Phosphatase (Cat. No. E2636) to detect Mouse IgG by indirect ELISA.
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | ambient |
| storage temp. | 2-8°C |
| technique(s) | dot blot: 1:300,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections | |
| indirect ELISA: 1:60,000 |
| Application: | ExtrAvidin® -Alkaline Phosphatase has been used in: • Dot blot • Immunohistochemistry (formalin-fixed, paraffin-embedded sections) • Indirect ELISA • Enzyme linked immunosorbent assay (ELISA) • Enzyme-linked immunosorbent spot (ELISPOT) analysis • Competitive ELISA |
| Biochem/physiol Actions: | Alkaline Phosphatase catalyzes the hydrolysis of esters to yield phosphoric acid. It is also facilitate the transport of substances across membranes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin has been conjugated to Alkaline Phosphatase and is used for various techniques. |
| Legal Information: | ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Solution in 0.05 M Tris-HCl buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin and 15 mM sodium azide |
| Preparation Note: | Affinity purified protein |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |