Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse
SIGMA/E2760 - clone 29.1, ascites fluid
Synonym: Anti-ERBB; Anti-ERBB1; Anti-ERRP; Anti-HER1; Anti-NISBD2; Anti-PIG61; Anti-mENA
MDL Number: MFCD00162282
Product Type: Chemical
Immunohistochemistry
Lung carcinoma labeled with Monoclonal Anti-Epidermal Growth Factor Receptor (Cat. No. E2760).
From R. Robinson, Univ. of Iowa, College of Med., Dept. of Pathology, Iowa City, IA.
Lung carcinoma labeled with Monoclonal Anti-Epidermal Growth Factor Receptor (Cat. No. E2760).
From R. Robinson, Univ. of Iowa, College of Med., Dept. of Pathology, Iowa City, IA.
Immunofluorescence
Photomicrograph of a mixed cell culture of an amelanotic melanoma cell line (Hs695T) and an epidermoid carcinoma cell line (A431). Cells were grown on coverslips, fixed and stained with Monoclonal Anti-EGF Receptor, clone no. 29.1 (Cat. No. E2760) – green fluorescence; and Hoechst No. 33258 (Cat. No. B 2883) – blue nuclear stain. Coverslips were photographed on a NIKON Microphot-FXA.
From G. MacKenzie, Immunogen, Inc., Cambridge, MA.
Photomicrograph of a mixed cell culture of an amelanotic melanoma cell line (Hs695T) and an epidermoid carcinoma cell line (A431). Cells were grown on coverslips, fixed and stained with Monoclonal Anti-EGF Receptor, clone no. 29.1 (Cat. No. E2760) – green fluorescence; and Hoechst No. 33258 (Cat. No. B 2883) – blue nuclear stain. Coverslips were photographed on a NIKON Microphot-FXA.
From G. MacKenzie, Immunogen, Inc., Cambridge, MA.
Western Blotting Whole extract of A431 cells were separated on SDS-PAGE and probed with Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in Mouse, Clone: 29.1 (Cat. No. P2859). The antibody was developed using 1:10,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1:6,000 antibody 2. 1:3,000 antibody 3. Negative Control
ELISA Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in Mouse, Clone: 29.1 (Cat. No. E2760), followed by 1:1,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917), was serially diluted to detect Epidermal Growth Factor Receptor by indirect ELISA.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 29.1, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | rabbit |
| species reactivity | pig, human, rat, mouse, canine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:10,000 using extract of the A431 cell line |
| western blot: 1:3,000 using extract of the A431 cell line | |
| UniProt accession no. | P00533  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) Western Blotting (1 paper) |
| Application: | May be used to purify EGF receptor, for structural and functional studies of the receptor and for investigation of cell transformation versus oncogenesis. |
| Application: | Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse has been used in • enzyme linked immunosorbent assay (ELISA) • immunoprecipitation • immunoaffinity • immunohistochemistry • western blotting |
| Biochem/physiol Actions: | Localizes blood type A-related determinants on various glycoproteins and glycolipids in addition to specific EGF receptor determinants (only observed in human tissue). Binds an external carbohydrate on the receptor and does not block EGF binding. |
| Biochem/physiol Actions: | The members of epidermal growth factor receptor (EGF R) or the ErbB receptor family have been identified as useful biomarkers and targets for cancer therapy. The EGFR family includes four receptor tyrosine kinases, EGF R (ErbB1), ErbB2 (Neu), ErbB3, and ErbB4. EGF R binds EGF and induces tyrosine phosphorylation leading to proliferation of cells. EGF R is present on many cell types of epithelial and mesenchymal lineages. EGF R is capable of binding transforming growth factor-α and heparin-binding EGF in addition to EGF. There are numerous effector molecule activated by EGF R that result in a variety of biological processes such as morphogenesis, cell motility, apoptosis, differentiation and organ repair and maintenance. Deregulation of EGF R signaling is implicated in progression of a wide variety of tumors, invasion and metastasis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The receptor for epidermal growth factor is an integral cell membrane protein of 170 kDa, which spans the membranes of a wide range of normal and malignant epithelial cells. A close similarity has been found between the sequence of the avian erythroblastic leukemia viral oncogene homolog (v-erb-B) and the cytoplasmic and transmembrane part of the EGF-R (truncated EGF-R). |
| Immunogen: | human carcinoma cell line A431, which expresses high levels of EGF receptors. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |