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Esterase from porcine liver

SIGMA/E2884 - ammonium sulfate suspension, ≥150 units/mg protein (biuret)

Synonym: Carboxyl esterase; Carboxylic-ester hydrolase; PLE

CAS Number: 9016-18-6
MDL Number: MFCD00131030
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-E2884-200UN 200 units
$78.60
1/EA
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45-E2884-1KU 1000 units
$85.50
1/EA
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45-E2884-5KU 5000 units
$229.00
1/EA
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45-E2884-20KU 20000 units
$663.00
1/EA
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biological source Porcine liver
form ammonium sulfate suspension
mol wt 168 kDa
Quality Level 300 
specific activity ≥150 units/mg protein (biuret)
storage temp. 2-8°C
Application: Esterase from porcine liver has been used to unmask the fluorescence in ultraviolet-visible spectroscopy.
Application: Porcine liver esterase is used to catalyze the hydrolysis of pentaacetyl catechin and epicatechin for use in pharmaceutical and industrial applications.

Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.
Application: The enzyme from Sigma has been used to develop an alternative gel-based protocol for screening for bead-bound catalytic activity in aqueous media. The product has also been used to investigate the properties of the compound L-valine-3-{8-[(E)-2-[3-methoxyphenyl)ethenyl]-7-methyl-1-propargylxanthine-3-yl}propyl ester hydrochloride (MSX-4, an aminoacid ester prodrug of the adenosine A2A receptor antagonist MSX-2).
Biochem/physiol Actions: Esterase acts on water-soluble carboxyl esters containing short chain fatty acids. Its functionality is attributed to the catalytic triad of Ser, His and Asp/Glu.
Biochem/physiol Actions: Esterase enzyme of bacteria and fungi seems to have several industrial applications. It is capable of catalyzing the hydrolysis and synthesis of ester bonds.
Biochem/physiol Actions: Pig liver esterase catalyzes enantioselective conversion of an ester to a carboxylic acid. The molecular weight is found to be 168 kDa. It is a serine enzyme with two active sites on each molecule, which dissociates into active half-molecules in the presence of dilute acid or concentrated salts.
General description: Esterase, also known as carboxyl ester hydrolase is a member of the hydrolase superfamily of enzymes.
Other Notes: One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.
Physical form: Suspension in 3.2 M (NH4)2SO4, pH 8
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
activity specific activity: ≥150 units/mg protein (biuret)
Storage Temp. 2-8°C
Enzyme Commission (EC) Number 3.1.1.1   ( BRENDA  | IUBMB  )
UNSPSC 12352204

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