Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse
SIGMA/E3138 - clone F4, ascites fluid
Synonym: Anti-EGFR
MDL Number: MFCD00162282
Product Type: Chemical
Immunohistochemistry
Frozen section of squamous cell carcinoma (vulva) stained with Monoclonal Anti-EGF Receptor, clone no. F4 (Cat. No. E3138) using the APAAP method.
From Dr. W.J. Gullick (London), reproduced from Cancer Res., 46, 285 (1986). Used with permission.
Frozen section of squamous cell carcinoma (vulva) stained with Monoclonal Anti-EGF Receptor, clone no. F4 (Cat. No. E3138) using the APAAP method.
From Dr. W.J. Gullick (London), reproduced from Cancer Res., 46, 285 (1986). Used with permission.
Western Blotting Whole extract of A431 Human epithelial carcinoma cells were separated on SDS-PAGE and probed with Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in Mouse, Clone: F4 (Cat. No. E3138). The antibody was developed using 1:5,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917). Lanes 1. 1:2,000 antibody 2. 1:4,000 antibody 3. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | F4, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable |
| immunohistochemistry (frozen sections): suitable | |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1:2,000 | |
| UniProt accession no. | P00533  |
| Application: | Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse has been used in: • western blotting • immunoprecipitation • immunocytochemistry • immunohistochemical staining |
| Biochem/physiol Actions: | EGFR is a transmembrane glycoprotein that binds to the EGF family. It is a pivotal regulator of cell proliferation, growth, differentiation and DNA repair after radiation-induced damage. Mutations in the gene encoding EGFR are associated with a number of malignancies including lung cancer and colorectal cancer. |
| Biochem/physiol Actions: | Epidermal growth factor receptor (EGF-R) is a tyrosine-specific protein kinase with the capacity to phosphorylate tyrosine residues located near its carboxy-terminus. |
| Biochem/physiol Actions: | This antibody recognizes specifically an intracellular domain of the EGF receptor. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The receptor for epidermal growth factor is an integral cell membrane protein of 170 kD, which spans the membranes of a wide range of normal and malignant epithelial cells. EGF-R have been identified in sarcomas, gliomas, breast, bladder, lung and gynecological tumors. The EGF-R has a half-life of approximately 10 hours in human fibroblasts, but in the presence of EGF this value is reduced to about 1 hour. |
| Immunogen: | synthetically produced residues 985-996 of the human EGF receptor. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 51111800 |