Catalase from bovine liver
SIGMA/E3289 - ≥20000 units/mg protein, lyophilized powder
Synonym: H2O2:H2O2 oxidoreductase
CAS Number: 9001-05-2
EC Number: 232-577-1
MDL Number: MFCD00081483
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: E3289-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: E3289-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: E3289-500MG
| composition | protein, ≥60% E405 |
| form | lyophilized powder |
| InChI | 1S/C9H10O3/c1-2-12-9(11)7 |
| InChI key | NUVBSKCKDOMJSU-UHFFFAOYSA |
| isoelectric point | 5.4 |
| mol wt | tetramer ~250 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| SMILES string | O(CC)C(=O)c1ccc(cc1)O |
| specific activity | ≥20000 units/mg protein |
| storage temp. | −20°C |
| Application: | Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalzes have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli. Catalase from bovine liver may be used:
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| Application: | Catalase from bovine liver has been used: • as a component of gloxy, which is one of the most popular oxygen-scavenging systems. • in single molecule fluorescence resonance energy transfer (FRET) measurements. • in cytochrome-C oxidase (COX) histochemistry to study the role of mitochondrial uncoupling protein 2 (UCP2) in limiting oxidative stress during glaucoma. • to pre-treat slide chambers for total internal reflection fluorescence (TIRF) microscopy. • in quantifying hydrogen peroxide release. • to help minimize the effects of reactive oxygen species (ROS) in cooperativity assay for studying doublecortin and microtubule interaction. |
| Biochem/physiol Actions: | Catalase has an ability to convert solar radiation into reactive oxidant species (ROS). It is involved in regulation of tissue peroxide levels. Mutation in catalase gene result in the progress of essential hypertension (EH). |
| Biochem/physiol Actions: | Catalase, an antioxidant enzyme found in all aerobic organisms, catalyzes the degradation of hydrogen peroxide, a byproduct of metabolic processes, into less harmful water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. Catalase enzyme uses either iron (Fe) or manganese (Mn) as cofactor, and are classified as Fe-CAT or Mn-CAT. |
| General description: | Research area: Cell Signaling Catalase is encoded by the gene mapped to human chromosome 11p13. Mammalian catalase is an antioxidant enzyme and is a member of hydroperoxidase family). The active enzyme is homotetrameric in nature. |
| General description: | Research Area: Cell Signaling Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms. |
| Other Notes: | One unit will decompose 1.0 micromole of hydrogen peroxide per minute at pH 7.0 at 25 deg C, while the hydrogen peroxide concentration falls from 10.3 to 9.2 millimolar. |
| Packaging: | 1 g in poly bottle |
| Packaging: | 100, 500 mg in poly bottle |
| Physical form: | Supplied as a lyophilized powder containing ≤0.2% thymol |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥20000 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.11.1.6 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |