EZview™ Red Protein G Affinity Gel
SIGMA/E3403
Synonym: EZview Affinity Gel; Protein G Affinity Gel
MDL Number: MFCD03456743
Product Type: Chemical
| analyte chemical class(es) | proteins (Immunoglobulins of various mammalian species) |
| capacity | ≥8 mg/mL, gel binding capacity (rabbit IgG) |
| form | liquid |
| Quality Level | 200  |
| storage temp. | −20°C |
| technique(s) | affinity chromatography: suitable |
| immunoprecipitation (IP): suitable |
| Application: | When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost. |
| Features and Benefits: | • Increased visibility - Red color reduces risk of incidental aspiration • Improved recovery of target protein by reduced accidental loss • Higher reproducibility - More consistent yields |
| General description: | EZ view Red Protein G Affinity Gel is Protein G covalently bonded to 4% Agarose beads. The affinity gel is used to bind the FC portion of the IgG of an antibody to samples, such as cell lysates and tissue, for purification of proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results. |
| Legal Information: | EZview is a trademark of Sigma-Aldrich Co. LLC |
| Packaging: | 1 mL in poly bottle |
| Physical form: | 1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon |
| Physical form: | 50% slurry in PBS with 50%Glycerol and 0.0015% |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |