Anti-Endothelin Receptor A antibody produced in rabbit
SIGMA/E3651 - affinity isolated antibody, lyophilized powder
Synonym: Anti-ET-A; Anti-ET1-Specific Endothelin Receptor
MDL Number: MFCD01092540
Product Type: Chemical
Immunohistochemistry Anti-Endothelin Receptor A: Cat. No. E3651: Immunohistochemical staining of paraffin embedded section of Rat lung using Anti-Endothelin Receptor A antibody, (1:100). ET-A is expressed both in respiratory epithelium (black arrows) and in respiratory smooth muscle (red arrows). Hematoxilin is used as the counterstain.
Immunohistochemistry Anti-Endothelin Receptor A: Cat. No. E3651: Immunohistochemical staining of Rat cerebellum using Anti-Endothelin Receptor A antibody (A), and Anti-ET-A antibody preincubated with the control peptide antigen (B). Cresyl violet is used as the counterstain.
Immunoblotting Anti-Endothelin Receptor A: Cat. No. E3651: Western blot analysis of Rat brain membranes. Lanes 1. Anti-Endothelin Receptor A antibody, (1:200). 2. Anti-Endothelin Receptor A antibody, preincubated with the control peptide antigen.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human, pig, chicken, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:100 using rat brain sections or rat lung paraffin embedded sections |
| western blot: 1:200 using rat brain membranes and ECL | |
| UniProt accession no. | P25101  |
| Application: | Anti-Endothelin Receptor A antibody produced in rabbit is suitable for immunohistochemistry at a dilution of 1:100 using rat brain sections or rat lung paraffin embedded sections and western blotting at a dilution of 1:200 using rat brain membranes and ECL. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) |
| Biochem/physiol Actions: | Endothelin receptor type A (ETA, ETAR) is a human G protein-coupled receptor that mediates vasoconstriction of blood vessels. It is involved in tumor cell invasion, survival and metastasis. ETAR mediates the promotion of ET-1 on colon cancer cell survival, invasion and MMP-2 expression by a PI3K-mediated mechanism. It plays a critical role in colon cancer metastasis. |
| Biochem/physiol Actions: | The epitope is highly conserved in human, swine, bovine and chicken ET-A. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | synthetic peptide corresponding to amino acids 413-426 of rat ET-A (with additional N-terminal cysteine). |
| Physical form: | Lyophilized from a solution of phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% sodium azide. |
| Hazard statements | H412 |
| Precautionary statements | P273 |
| Hazard Codes | Xn |
| Risk Statements | 21/22-52/53 |
| Safety Statements | 22-36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |