Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid

SIGMA/E3889 - Molecular Biology, ≥97.0%

Synonym: EGTA; Egtazic acid; Ethylene-bis(oxyethylenenitrilo)tetraacetic acid; Glycol ether diamine tetraacetic acid

CAS Number: 67-42-5
Empirical Formula (Hill Notation): C₁₄H₂₄N₂O₁₀
Molecular Weight: 380.35
EC Number: 200-651-2
MDL Number: MFCD00004291
Linear Formula: [-CH₂OCH₂CH₂N(CH₂CO₂H)₂]₂
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-E3889-10G	10 g	$105.00 1/EA	Add To Favorites
45-E3889-25G	25 g	$184.00 1/EA	Add To Favorites
45-E3889-100G	100 g	$497.00 1/EA	Add To Favorites
45-E3889-500G	500 g	$1790.00 1/EA	Add To Favorites

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This image depicts SKU: E3889-100G

Properties
Descriptions
Safety Info.
Basic Data

assay	≥97.0%
foreign activity	DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected
form	powder
grade	Molecular Biology
InChI	1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)
InChI key	DEFVIWRASFVYLL-UHFFFAOYSA-N
loss	≤1.0% loss on drying
mp	241 °C (dec.) (lit.)
Quality Level	400
reaction suitability	reagent type: chelator
SMILES string	OC(=O)CN(CCOCCOCCN(CC(O)=O)CC(O)=O)CC(O)=O

Application:	A chelating agent useful for the determination of calcium in the presence of magnesium.
Application:	Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid has been used: • in Dulbecco′s phosphate-buffered saline (DPBS) to induce the weakening of cell-to-cell junctions • as an extracellular calcium chelator to study the role of calcium in axonal mitochondria degeneration • as a component in lysis buffer for lysing mouse mucosal cells for western blot analysis
Biochem/physiol Actions:	Ethylene glycol tetraacetic acid (EGTA) can chelate calcium (Ca²⁺) at a ratio of 1:1. It serves as an anti-coagulant when dissolved at 1 g per 100 ml of blood. EGTA can prevent the vesicular release in the nanodomain of single Ca²⁺ channels. This metal chelator can inhibit the activity of pseudolysin.
Biochem/physiol Actions:	Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid A is a chelating agent used for the determination of calcium in the presence of magnesium.
Packaging:	10, 25, 100, 500 g in poly bottle

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2

Purity	≥97.0%
mp	241 °C (dec.) (lit.)
UNSPSC	12352106

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