Monoclonal Anti-Elastin antibody produced in mouse
SIGMA/E4013 - clone BA-4, ascites fluid
MDL Number: MFCD00164587
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded sheep arterial tissue with Monoclonal Anti-Elastin, Clone: BA-4 (Cat. No. E4013) diluted to 1:5,000 using biotin/ExtrAvidin®-Peroxidase.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | BA-4, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 67.5 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, goat, sheep, guinea pig, pig, canine, feline, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:5,000 using sheep arterial tissue |
| microarray: suitable | |
| UniProt accession no. | P15502  |
| Application: | Monoclonal Anti-Elastin antibody produced in mouse has been used in: • immunohistochemistry • immunostaining • immunoblotting |
| Biochem/physiol Actions: | Elastin is encoded by the ELN gene in humans. It is a polymeric protein in connective tissue that has exceptional properties of extension, elastic recoil to the extracellular matrix and allows many tissues in the body to resume their shape after stretching or contracting. Elastin is a self-assembling, extracellular-matrix protein providing tissue elasticity and is predominantly comprised of cross-linked tropoelastin. It plays an important role in the atherosclerotic process. The conformational disorder is caused due to a constitutive feature of elastin structure and function. |
| Biochem/physiol Actions: | Elastin undergoes changes of morphology with aging and in many disease states. For example, abnormal accumulation of connective tissue in blood vessels contributes to alterations in vascular physiology associated with diseases such as hypertension and atherosclerosis. Several heritable diseases such as Marfan syndrome, pseudoxanthoma elasticum and the Buschke-Ollendorf syndrome may result from molecular defects of elastin. |
| Biochem/physiol Actions: | The antibody binds to insoluble elastin, α-elastin, tropoelastin (the soluble non-cross linked precursor of elastin) and to peptide fragments generated by proteolytic digestion of insoluble elastin. In tropoelastin preparations, three immunoreactive bands have been observed at 67.5 kDa, 65 kDa and 62 kDa corresponding to the three elastin isoforms. The antibody is specific for an epitope composed of the repeated sequence in elastin: Val-Gly-Val-Ala-Pro-Gly. This epitope has been shown to be a chemoattractant for fibroblasts and monocytes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Elastin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Elastin is an important polymeric protein of connective tissue. It is synthesized and secreted as a soluble single-chain protein (tropoelastin) that undergoes numerous post-ribosomal modifications prior to the organization of the elastic fiber in the extracellular space. |
| Immunogen: | bovine α-elastin. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |