Endotoxin Removal solution
SIGMA/E4274 - Remove lipopolysaccharides (LPS) from plasmid DNA preparations
Synonym: ET removal buffer; ET removal reagent; ET removal solution; endo removal buffer; endo removal reagent; endo removal solution; endotoxin contamination removal; endotoxin removal buffer; endotoxin removal reagent; plasmid purification endo; plasmid purification endo free
Product Type: Chemical
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| Quality Level | 200  |
| Application: | Endotoxin Removal solution has been used to remove lipopolysaccharide (LPS) from Baker’s yeast RNA. |
| Biochem/physiol Actions: | The reagent relies on the micellar properties of Triton X-114 to remove endotoxins from solution. When warmed above 20 °C, micelles aggregate into a separate phase that sequesters amphiphilic solutes, including lipopolysaccharides. The hydrophilic DNA remains in the aqueous phase. |
| Preparation Note: | 1 volume treats 5 volumes of DNA solution (≤1mg/ml) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12352200 |