Anti-ELKS antibody, Mouse monoclonal

SIGMA/E4531 - clone ELKS-30, purified from hybridoma cell culture

Synonym: Anti-KIAA1081

MDL Number: MFCD08705584
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-E4531-200UL	200 µL	$719.00 1/EA	Add To Favorites

Immunoblotting Whole extract of human HeLa cells was separated on SDS-PAGE, blotted with Monoclonal Anti-ELKS Clone: ELKS-30 (Cat. No. E4531) and developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 1 μg/mL antibody 2. 0.5 μg/mL antibody

Properties
Descriptions
Safety Info.
Basic Data

antibody form	purified immunoglobulin
antibody product type	primary antibodies
biological source	mouse
clone	ELKS-30, monoclonal
concentration	~2 mg/mL
conjugate	unconjugated
form	buffered aqueous solution
isotype	IgG2a
mol wt	antigen ~120 kDa
shipped in	dry ice
species reactivity	rat, chicken, bovine, human, mouse
storage temp.	−20°C
target post-translational modification	unmodified
technique(s)	immunocytochemistry: suitable
	immunoprecipitation (IP): suitable
	microarray: suitable
	western blot: 0.5-1 μg/mL using HeLa cell extract
UniProt accession no.	Q8IUD2

Application:	Monoclonal Anti-ELKS antibody produced in mouse has been used in: • enzyme-linked immunosorbent assay (ELISA) • fluorescence immunohistochemistry • immunocytochemistry • immunoblotting
Biochem/physiol Actions:	ELKS/RAB6-interacting/CAST family member 1 is a protein encoded by the ERC1 gene in humans. It encodes for a synaptic factor and is found in the smallest region of overlap. ERC1 encodes a regulator of neurotransmitter release and acts as the best gene candidate contributing to this phenotype as well as to the autism spectrum disorder ASD. ERC1a (an isoform of ERC1) is associated with other proteins for effective migration and tumor cell invasion by stabilizing the activity at the cell front. Depletion of any of the associated protein negatively affects invasion, migration on extracellular matrix, lamellipodial persistence and the internalization of active integrin β1 receptors needed for adhesion turnover at the front of the cell.
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description:	ELKS/RAB6-interacting/CAST family member 1 is a protein encoded by the ERC1 gene in humans. It encodes for a synaptic factor and is found in the smallest region of overlap.
General description:	Monoclonal Anti-ELKS (mouse IgG2a isotype) is derived from the hybridoma ELKS-30 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human ELKS. ELKS, also known as KIAA1081, is an essential regulatory subunit of the IKK complex. It was named ELKS since 44.1% of the sequence is composed of glutamic acid (E), leucine (L), lysine (K) and serine (S) residues. The protein contains 948 amino acids with nine -helical coiled-coil domains including periodic heptad repeats that predict dimer formation. Its highest expression is in heart, placenta, lung, brain, thyroid and testis.
Physical form:	Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	−20°C
UNSPSC	12352203

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