Epidermal Growth Factor, human, animal component free
SIGMA/E5036 - EGF, recombinant, expressed in Escherichia coli, >97% (SDS-PAGE)
Synonym: Epidermal Growth Factor human; EGF
CAS Number: 62253-63-8
MDL Number: MFCD00132565
Product Type: Chemical
Epidermal
Growth Factor Receptor (EGFR) Pathway Research areas:
Cancer biology, Cell proliferation, Metabolism. Disease areas:
Breast, lung, stomach, gastrointestinal cancers, chronic kidney diseases,
glioblastomas.
SDS-PAGE The purity of recombinant Human EGF (Cat. No. E5036) was determined by SDS-PAGE of reduced EGF and staining with Coomassie Blue.
Bioassay for Epidermal Growth Factor The biological activity of recombinant, human EGF (Cat. No. E5036) is measured by its ability to stimulate the mouse fibroblast cell line BALB/3T3. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.
| assay | >97% (SDS-PAGE) |
| biological source | human |
| color | white |
| form | lyophilized powder |
| impurities | ≤1 EU/μg Endotoxin |
| InChI | 1S/C270H401N73O83S7/c1-24 |
| InChI key | GVUGOAYIVIDWIO-UFWWTJHBSA |
| mol wt | ~6 kDa |
| packaging | pkg of 200 and 500 μg |
| potency | 0.08-0.8 ng/mL EC50 |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| SMILES string | S(CC[C@H](NC(=O)[C@@H](NC |
| solubility | water: soluble 0.190-0.210, clear, colorless |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| UniProt accession no. | P01133  |
| Application: | Epidermal Growth Factor, human, animal component free has been used: • as a supplement in in LHC-8 medium to culture liver cell lines • in the fetal bovine serum (FBS)-Dulbecco′s modified essential medium (DMEM) /F12 medium for primary culture of human glioma cells • as an additive in the conditional medium of normal fibroblasts (NFs) to study its effect on the migration and invasion of endometrial cancer (EC) cells • as a component in tumorsphere medium |
| Biochem/physiol Actions: | Epidermal growth factor (EGF) helps to induce cell growth, proliferation and differentiation. It participates in the repairing of renal tissues in the kidney. It promotes the reabsorption of magnesium with the help of the transient receptor potential cation channel 6 (TRMP6). EGF is known to participate in the pathophysiology of drug-induced renal magnesium loss. |
| General description: | Epidermal Growth Factor (EGF) is a small mitogenic polypeptide (∼6 kDa), which is present in many mammalian species and is distributed throughout a wide number of tissues and body fluids.† Human EGF is identical to β-urogastrone, a polypeptide which was recognized and isolated on the basis of its ability to inhibit gastric acid secretion.† EGF is a member of a growth factor family, which is characterized by the presence of 6 conserved cysteine motifs that form three disulfide bonds. The location of 3 intrachain disulfide bonds in recombinant human EGF is identical to that of mouse EGF.† EGF is homologous to a sequence contained in a 19 kDa protein of vaccinia virus,† which appears to utilize the EGF receptor to gain entry into cell† EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa cells, and SV40-3T3 cells.† |
| General description: | Epidermal growth factor (EGF) is synthesized in Henle′s loop and the distal convoluted tubule in the kidney. It is also produced in salivary glands and duodenum. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >97% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |