Epidermal Growth Factor from mouse
SIGMA/E5160 - EGF
Synonym: Mouse EGF; Mouse Epidermal Growth Factor
Product Type: Chemical
SDS-PAGE Epidermal Growth Factor from Mouse (Cat. No. E5160) was assessed on SDS-PAGE.
Epidermal
Growth Factor Receptor (EGFR) Pathway Research areas:
Cancer biology, Cell proliferation, Metabolism. Disease areas:
Breast, lung, stomach, gastrointestinal cancers, chronic kidney diseases,
glioblastomas.
Bioassay EC50 is defined as the effective concentration of EGF that elicits a 50% attachment factor in cell based bioassay.
| assay | ≥90% (SDS-PAGE) |
| biological source | mouse |
| color | white |
| form | lyophilized powder |
| impurities | ≤1 EU/μg endotoxin (Protein) |
| mol wt | ~6 kDa |
| packaging | pkg of 100 μg |
| potency | 0.05-1 ng/mL ED50/EC50 |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| solubility | water: soluble 0.10 mL, clear, colorless |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P01132  |
| Application: | Epidermal Growth Factor from mouse has been used as a basal (N2B27) medium supplement for culturing differentiated mouse embryonic stem (E14) cells. |
| Biochem/physiol Actions: | EGF (epidermal growth factor) is involved in cellular proliferation, differentiation, and survival. Moreover, it was found to affect various biological activities like angiogenesis, inhibition of gastric acid secretion, modulation of the synthesis of a number of hormones, synthesis and turn-over of proteins of the extracellular matrix, calcium release from bone tissue (thus promoting bone resorption), chemoattraction of fibroblasts and epithelial cells, and alone or in combination with other cytokines, mediation of wound healing processes. EGF is mitogenic for a large variety cell types, including fibroblasts, epithelial cells, endothelial cells, chondrocytes, and SV40-3T3 cells. |
| General description: | Epidermal Growth Factor (EGF) is a small mitogenic polypeptide (∼6kDa) present in many mammalian species and distributed throughout a wide number of tissues and body fluids. Four ErbB (HER) family receptor tyrosine kinases, including EGFR/ErbB1, ErbB2, ErbB3, and ErbB4 mediate responses to EGF family members. Human and mouse EGFs are very similar, but not identical in their physical and chemical properties. Of the 53 amino acid residues comprising each of the two polypeptides, 37 are common to both molecules, and three disulfide bonds are formed in the same relative positions. |
| Physical form: | The product is lyophilized from a 0.2 μm-filtered solution of phosphate buffered saline (PBS), pH 7.4. |
| RIDADR | UN 3077 9 / PGIII |
| WGK Germany | WGK 1 |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |