Erythromycin
SIGMA/E5389 - BioReagent, suitable for cell culture
Synonym: E-Mycin; Erythrocin
CAS Number: 114-07-8
Empirical Formula (Hill Notation): C37H67NO13
Molecular Weight: 733.93
EC Number: 204-040-1
MDL Number: MFCD00084654
Linear Formula: C37H67NO13
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: E5389-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: E5389-5G
| antibiotic activity spectrum | Gram-negative bacteria |
| Gram-positive bacteria | |
| color | white |
| form | powder |
| impurities | ≤0.1 EU/mg endotoxin |
| InChI | 1S/C37H67NO13/c1-14-25-37 |
| InChI key | ULGZDMOVFRHVEP-RWJQBGPGSA |
| mode of action | protein synthesis | interferes |
| mp | 133 °C |
| potency | ≥850 μg per mg |
| product line | BioReagent |
| Quality Level | 200  |
| SMILES string | CC[C@H]1OC(=O)[C@H](C)[C@ |
| solubility | 2 M HCl: 50 mg/mL (Stock solutions should be stored at 2-8 °C. Stable at 37 °C for 3 days.) |
| ethanol: soluble (Stock solutions should be stored at 2-8 °C. Stable at 37 °C for 3 days.) | |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Erythromycin has been used: • as a supplement for nutrient broth medium for culturing green fluorescent protein (GFP)- expressing E. coli • as a model drug to determine small intestinal (SMI) microtissue viability using the MTT assay{254 • as an antibiotic to study the treatment strategies of chronic infections |
| Biochem/physiol Actions: | Mode of Action: Erythromycin acts by inhibiting elongation at the transpeptidation step, specifically aminoacyl translocation from the A-site to P-site by binding to the 50s subunit of the bacterial 70s rRNA complex. Antimicrobial Spectrum: This product acts against both gram-negative and gram-positive bacteria. |
| Disclaimer: | This product is stable in solution at 37°C for 3 days. Stock solutions should be stored at 2-8°C. |
| General description: | Chemical structure: macrolide |
| General description: | Erythromycin is an antibiotic produced by growth of certain strains of Streptomyces erythreus. This product is composed largely of erythromycin A with small amounts of erythromycins B and C and is recommended for concentration at 100 mg/L. Concentrations between 50 and 200 mg/L have also proven effective in controlling bacterial growth. Erythromycin has been used as a motilin receptor agonist, to block respiratory glycoconjugate secretion in human airways in vitro, and for selecting plasmid-cured and recombinant lactococcus lactis MG1363 strains. |
| Packaging: | 1, 5 g in poly bottle |
| Preparation Note: | This product is soluble in water at 2 mg/mL, with a 0.067% solution in water yielding a pH of 8.0-10.5. It is also soluble in ethanol at 50 mg/mL, yielding a clear, colorless to faint yellow solution. It is freely soluble in alcohol, acetone, chloroform, acetonitrile and ethyl acetate but forms salts with acids. All solutions should be protected from light. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 133 °C |
| UNSPSC | 12352207 |