EZview™ Red Streptavidin Affinity Gel
SIGMA/E5529
Product Type: Chemical
| capacity | ~10 μg(biotin per ml of packed gel) |
| form | suspension |
| matrix | 4% agarose |
| pH | 7.2 |
| Quality Level | 200  |
| shelf life | 1 yr |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| technique(s) | western blot: suitable |
| Application: | Sutitable for use with immunoprecipitation, western blotting,and enzyme assays. |
| Application: | When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview™ resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost. |
| Features and Benefits: | • Increased visibility - Red color reduces risk of incidental aspiration • Improved recovery of target protein by reduced accidental loss • Higher reproducibility - More consistent yields |
| General description: | The EZ View Red Streptavidin Affinity gel is composed of streptavidin, covalently attached to cyanogen bromide-activated 4% agarose beads. It is designed to capture (pull-down) the biotinylated target molecules, such as proteins, peptides, antibodies, nucleic acids, lectins, receptors and ligands. |
| Legal Information: | EZview is a trademark of Sigma-Aldrich Co. LLC |
| Packaging: | 1 mL in poly bottle |
| Physical form: | 1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106500 |