Anti-eIF4E antibody produced in rabbit
SIGMA/E5906 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-CBP; Anti-Eukaryotic intiation factor 4E; Anti-MGC111573; Anti-eIF4E1; Anti-eIF4EL1; Anti-eIF4F
MDL Number: MFCD01633313
Product Type: Chemical
Immunofluorescence NIH-3T3 Mouse fibroblasts cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.1% Triton X-100. Fixed cells were stained with 5 μg/mL Anti-eIF4E antibody produced in Rabbit (Cat. No. E5906) followed by 1:500 Anti-Rabbit IgG (whole molecule)-Cy3 antibody. Picture B: cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Analysis of lysates from several cell lines, using Anti-eIF4E antibody: Cell extracts were separated on SDS-PAGE and blotted with Rabbit Anti-eIF4E (Cat. No. E5906), at a concentration of 0.5 μg/mL. The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescence substrate. Lysates from the following cell lines were loaded: Lane 1, HEK-293T; Lane 2, HEK-293T transfected with eIF4E cDNA; Lane 3, HeLa; Lane 4, MCF7; Lane 5, U2OS; Lane 6, Jurkat; Lane 7, HepG2; Lane 8, A431; Lane 9, HL60; Lane 10, K562; Lane 11, NIH3T3; Lane 12, PC12; Lane 13, RAT1
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 25 kDa |
| shipped in | dry ice |
| species reactivity | rat (predicted), human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 5-10 μg/mL using paraformaldehyde-fixed 3T3 cells |
| immunoprecipitation (IP): 2-5 μL using HeLa cell lysates | |
| western blot: 0.5-1 μg/mL using HeLa cell lysates | |
| UniProt accession no. | P06730   |
| Application: | Anti-eIF4E antibody produced in rabbit has been used in immunoblotting |
| Biochem/physiol Actions: | The translational factor eukaryotic translation initiation factor 4EeIF4E regulates the recruitment of mRNAs to the ribosome. Its interaction with mRNA cap structure enhances its binding to eIF4G and 40S ribosomal subunit. eIF4Eplays a role in diverse biological processes including embryonic development, cell cycle progression, synaptic plasticity, and cancer. eIF4E is subjected to tight regulation both at the level of its expression and by control of its activity by phosphorylation mitogen-activated protein kinase (MAPK) interacting protein kinases (by the Mnk kinase). Human P bodies contain the cap-binding protein eIF4E and the related factor eIF4E-transporter (eIF4E-T; eIF4ENIF1), suggesting novel roles for these proteins in targeting mRNAs for 5′ to 3′ degradation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The translational factor eukaryotic translation initiation factor 4E (eIF4E), also known as mRNA cap-binding protein(CBP) or EIF4E1 is a central component of translation initiation and regulation in eukaryotic cells. eIF4E exists as both a free form and as part of a multiprotein complex termed eIF4F. Although mainly expressed in the cytoplasm, a fraction of eIF4E localizes to the nucleus, and some of the biological effects of eIF4E might be effected by a nuclear function. |
| Immunogen: | synthetic peptide corresponding to amino acids 21-37 of human eIF4E, conjugated to KLH via a C-terminal added cysteine residue. The sequence is conserved in human, rat, and mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |