Enolase from baker’s yeast (S. cerevisiae)
SIGMA/E6126 - lyophilized powder, ≥50 units/mg protein
Synonym: 2-Phospho-D-glycerate hydro-lyase; Phosphopyruvate hydratase
CAS Number: 9014-08-8
EC Number: 232-754-3
MDL Number: MFCD00131019
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: E6126-12.5KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: E6126-2.5KU
| composition | Protein, 50-80% modified Warburg-Christian |
| form | lyophilized powder |
| mol wt | 93 kDa |
| Quality Level | 200  |
| solubility | 15 mM Tris HCl, pH 7.4: soluble 1.0 mg/mL, clear |
| specific activity | ≥50 units/mg protein |
| storage temp. | −20°C |
| Application: | Enolase from baker′s yeast has been used in a study to investigate the contribution of the antibodies response induced by a low virulent Candida albicans strain in protection against systemic candidiasis. Enolase from baker′s yeast has also been used in a study to investigate the role of metal ions in catalysis by enolase. |
| Application: | Enolase from baker′s yeast (S. cerevisiae) has been used as a supplement in assay buffer for phosphoglycerate mutase (PGM) enzyme assays. |
| Application: | The enzyme from Sigma has been used as an antigen during ELISA. the study used human granulocyte proteins to identify and characterize autoantibodies against catalase and α-enolase in patients with primary sclerosing cholangitis. It has been used to study temperature- and denaturant-induced yeast enolase denaturation using fourier transform infrared spectroscopy. It has also been used along with other proteins to study gradient chromatofocusing-mass spectrometry; a new technique for protein analysis. |
| Biochem/physiol Actions: | Enolase is a metalloenzyme that catalyzes the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Enolase is essential for both glycolysis and gluconeogenesis. |
| Biochem/physiol Actions: | Enolase plays a key role in glycolysis and gluconeogenesis. Enolase, found in the nucleus of mammalian cells plays a key role in transcriptional modulation of genes, which participates in morphological transformation and cell proliferation. |
| Biochem/physiol Actions: | The phosphorylated tyrosine residue present in yeast enolase forms a substrate for phosphorylation by tyrosine protein kinase. Apart from Mg2+, the enzyme can be activated by Zn2+, Mn2+, and Cd2+. |
| General description: | Enolase (2-phosphoglycerate hydratase) is a cytosolic enzyme. This cytosolic metalloenzyme is encoded by three homologous genes in mammals. Enolase the from baker′s yeast is a homodimer containing two bound Mg2+ ions. The peptide consists of 436 amino acids and contains a single cysteine residue and corresponds to a molecular weight of 93 kDa. Two of the active site components include His191 and Arg414. Enolase is seen in archaea, bacteria and eukaryotes. |
| Other Notes: | One unit will convert 1.0 μmole of 2-phosphoglycerate to phospho(enol)pyruvate per min at pH 7.4 at 25 °C. |
| Packaging: | 500, 2500, 12500 units in poly bottle |
| Physical form: | Lyophilized powder containing Tris buffer salts |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥50 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 4.2.1.11 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |