Anti-Endothelial Cell Protein C Receptor antibody, Rat monoclonal
SIGMA/E6280 - clone RCR-252, purified from hybridoma cell culture
Synonym: Anti-EPCR
MDL Number: MFCD04118502
Product Type: Chemical
Flow Cytometry 1×106 HUVEC cells were labeled with 1 μg/test Anti-Endothelial Cell Protein C Receptor antibody, Rat monoclonal, Clone: RCR-252 (Cat. No. E6280) followed by Anti-Rat IgG (whole molecule)-FITC antibody produced in Rabbit (Cat. No. F1763). Red histogram: negative control without first antibody; Green histogram: antibody specific staining.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | RCR-252, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen 49 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 5-20 μg/mL using HUVEC cells |
| microarray: suitable | |
| UniProt accession no. | Q9UNN8  |
| Application: | Anti-endothelial cell protein C receptor antibody, rat monoclonal has been used in flow cytometry (FACS analysis) and in blocking the binding of the antigen presenting cell (APC) ligand to the EPCR. |
| Biochem/physiol Actions: | Endothelial protein C receptor (EPCR) is involved in regulation of the cytoprotective and anticoagulant pathways of protein C. EPCR plays an important role in regulating the inflammatory response. It is also identified as an endothelial receptor for specific P. falciparum erythrocyte membrane protein 1 (PfEMP1) subtypes. EPCR is associated with an increased risk of venous thromboembolism (VTE). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-endothelial cell protein C receptor antibody, rat monoclonal (EPCR) (rat IgG1 isotype) is derived from the RCR-252 hybridoma produced by the fusion of mouse SP2/0 myeloma cells and cells isolated from the superficial inguinal lymph nodes from Wister rats immunized with human EPCR-positive RE-1 cells. Endothelial protein C receptor (EPCR), also known as activated protein C receptor (APC receptor) is a protein encoded by the PROCR gene in humans. It is predominantly expressed in endothelial. The EPCR is also expressed in small vessels such as capillaries of the alveolar wall in the lung. EPCR is a member of the CD1/major histocompatibility complex superfamily. |
| Immunogen: | human EPCR-positive RE-1 cells. |
| Packaging: | 200 μL in glass bottle |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |