Anti-eIF2Bβ (N-terminal) antibody produced in rabbit
SIGMA/E6282 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-EIF-2B; Anti-EIF2B-Beta; Anti-EIF2BB; Anti-Eukaryotic translation initiation factor 2B, Beta; Anti-Eukaryotic translation initiation factor 2B, subunit 2
Product Type: Chemical
Immunofluorescence Anti-eIF2Bb: Cat. No. E6282: Immunofluorescence of HUVEC cells using Anti-eIF2Bb (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used.
Immunofluorescence Graph Anti-eIF2Bb: Cat. No. E6282: Intensity analysis of Anti-eIF2Bb staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.
Western Blotting AT3B1 cell lysates were separated on SDS-PAGE and probed with Anti-eIF2Bβ (N-terminal) antibody produced in Rabbit (Cat. No. E6282). The antibody was developed using 1:30,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 4 μg/mL antibody 2. 2 μg/mL antibody 3. 1 μg/mL antibody 4. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~39 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 3-5 μg using K562 cell lysates |
| indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 cells over-expressing human eIF2Bβ | |
| indirect immunofluorescence: suitable | |
| western blot: 2-4 μg/mL using K562 or AT3B1 cell lysates | |
| UniProt accession no. | P49770  |
| Application: | Anti-eIF2Bβ (N-terminal) antibody produced in rabbit is suitable for the following applications: • Immunoprecipitation at a concentration of 3-5μg using K562 cell lysates • Indirect immunofluorescence (at a concentration of 2-5μg/mL using paraformaldehyde-fixed NIH-3T3 cells over-expressing human eIF2Bβ) • Western blotting at a concentration of 2-4μg/mL using K562 or AT3B1 cell lysates Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if |
| Biochem/physiol Actions: | Anti-eIF2BNβ-terminal) specifically recognizes human, mouse, and rat eIF2Bβ. |
| Biochem/physiol Actions: | Recent studies have linked inherited mutations in any of the five eIF2B subunits to a fatal human disorder known as childhood ataxia with central nervous system hypomyelination (CACH) or vanishing white matter (VWN) disease. The eIF2B -α, -β and - δ subunits downregulate eIF2B activity in response to the phosphorylation of eIF2 on Ser51. |
| Biochem/physiol Actions: | Translation initiation factor eIF-2B subunit β is a protein encoded by EIF2B2 gene in humans. It is a five-subunit complex that catalyzes guanine nucleotide exchange on eIF2. It is associated with the cause of autosomal recessive disorders. Autosomal recessive inherited mutations in each of the five eukaryotic initiation factor 2B (eIF2B) subunits are known to cause white matter abnormalities called eIF2B-related disorders. |
| Disclaimer: | Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Translation initiation factor eIF-2B subunit beta eIF2B β is a component of the regulatory subcomplex of eIF2B along with the eIF2Bα and -δ subunits. eIF2Bβ gene is mapped to human chromosome 14q24.3. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |