Anti-Endothelial Cell Protein C Receptor antibody, Rat monoclonal
SIGMA/E6405 - clone RCR-379, purified from hybridoma cell culture
Synonym: Anti-EPCR; Monoclonal Anti-Endothelial Cell Protein C Receptor antibody produced in rat
MDL Number: MFCD04118502
Product Type: Chemical
Flow Cytometry 1×106 HUVEC cells were labeled with 1 μg/test Anti-Endothelial Cell Protein C Receptor antibody, Rat monoclonal, Clone: RCR-379 (Cat. No. E6405) followed by Anti-Rat IgG (whole molecule)-FITC antibody produced in Rabbit (Cat. No. F1763). Red histogram: negative control without first antibody; Green histogram: antibody specific staining.
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | RCR-379, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 5-20 μg/mL using HUVEC cells |
| UniProt accession no. | Q9UNN8  |
| Application: | Monoclonal Anti-Endothelial Cell Protein C Receptor antibody produced in rat is suitable for flow cytometry at a concentration of 5-20μg/mL using HUVEC cells. |
| Application: | Monoclonal Anti-Endothelial Cell Protein C Receptor antibody produced in rat may be used in immunohistochemistry. |
| Biochem/physiol Actions: | Endothelial protein C receptor (EPCR) also known as activated protein C receptor (APC receptor) is involved in regulation of the cytoprotective and anticoagulant pathways of protein C. EPCR is a member of the CD1/major histocompatibility complex superfamily and plays an important role in regulating the inflammatory response. It is also identified as an endothelial receptor for specific P. falciparum erythrocyte membrane protein 1 (PfEMP1) subtypes. EPCR is associated with an increased risk of venous thromboembolism (VTE). |
| Biochem/physiol Actions: | Monoclonal Anti-Endothelial Cell Protein C Receptor (EPCR) recognizes human EPCR (49 kDa). |
| Biochem/physiol Actions: | The EPCR protein also performs other functions, like binding to proteinase. The complex then binds to macrophage 1 antigen (MAC-1) on activated neutrophils and inhibits their adhesion to activated endothelium |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-endothelial cell protein C receptor (EPCR) (rat IgG2a isotype) is derived from the RCR379 hybridoma produced by the fusion of mouse SP2/0 myeloma cells and lymphatic cells isolated from the superficial inguinal lymph nodes from Wister rats immunized with human EPCR-positive RE-1 cells. Endothelial protein C receptor (EPCR), also known as activated protein C receptor (APC receptor), is a protein encoded by the PROCR gene in humans and is mapped to the locus 20q11.22. EPCR is a type 1 transmembrane glycoprotein containing two domains in the extracellular region that are homologous to the α1 and α2 domains of CD1/ major histocompatibility complex class 1 (MHC) class 1 molecules. It is expressed exclusively in endothelial cells and small vessels such as capillaries of the alveolar wall in the lung. |
| Immunogen: | human EPCR-positive RE-1 cells. |
| Packaging: | 200 μL in glass insert |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |