EZview™ Red Glutathione Affinity Gel
SIGMA/E6406
MDL Number: MFCD05665380
Product Type: Chemical
| analyte chemical class(es) | proteins (GST) |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| technique(s) | immunoprecipitation (IP): suitable |
| protein purification: suitable |
| Application: | The product is used for affinity capture, molecular pull-down, or IP of proteins containing glutathione binding sequences, such as glutathione S-transferase, glutathione peroxidase and glyoxalase I. |
| Application: | When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview™ resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost. |
| Features and Benefits: | • Increased visibility - Red color reduces risk of incidental aspiration • Improved recovery of target protein by reduced accidental loss • Higher reproducibility - More consistent yields |
| General description: | EZviewä Red Glutathione Affinity Gel is a highly visible, red colored glutathione agarose affinity gel. The affinity resin is composed of glutathione covalently attached through the sulfur to epoxy-activated 4% cross-linked agarose beads, resulting in a 12-atom spacer. |
| Legal Information: | EZview is a trademark of Sigma-Aldrich Co. LLC |
| Packaging: | 1 mL in poly bottle |
| Physical form: | 1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106500 |