Anti-eIF2Bε antibody produced in rabbit
SIGMA/E6407 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-Eukaryotic translation initiation factor 2B, ε; Anti-Eukaryotic translation initiation factor 2B, subunit 5; Anti-eIF-2B GDP-GTP exchange factor subunit epsilon
Product Type: Chemical
Immunofluorescence Anti-eIF2Be: Cat. No. E6407: Immunofluorescence of HUVEC cells using Anti-eIF2Be (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used.
Immunofluorescence Localization of eIF2Bε in NIH-3T3 cytoplasm. Cells were stained with Rabbit Anti-eIF2Bε (Cat. No. E6407) at a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, Cy3 conjugate, and counterstained with DAPI (blue) to stain nuclei (x40 magnification).
Immunofluorescence Graph Anti-eIF2Be: Cat. No. E6407: Intensity analysis of Anti-eIF2Be staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.
Immunoblotting Lysates from various cell lines were separated on SDS-PAGE and probed with 1:500 dilution of Rabbit Anti-eIF2Bε (Cat. No. E6407). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminiscence substrate. Lanes: 1. 293-T; 2. HeLa; 3. MCF-7; 4. A549; 5. A431; 6. 3T3; 7. C2; 8. RAT1; 9. PC12
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~80 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 1:500-1:1,000 using paraformaldehyde fixed NIH-3T3 cells. |
| indirect immunofluorescence: suitable | |
| western blot: 1:500-1:1,000 using Rat1 or HEK-293T cell lysates. | |
| UniProt accession no. | Q13144  |
| Application: | Anti-eIF2Bε antibody produced in rabbit is suitable for indirect immunofluorescence (at a dilution of 1:500-1:1,000 using paraformaldehyde fixed NIH-3T3 cells) and western blotting at a dilution of 1:500-1:1,000 using Rat1 or HEK-293T cell lysates. Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if . |
| Biochem/physiol Actions: | Translation initiation factor eIF-2B subunit ε is a protein encoded by the EIF2B5 gene in humans. The eIF2B is composed of five subunits α, β, γ, δ and ε, within which the ε subunit is responsible for catalyzing the guanine exchange reaction. Defect in any of these 5 subunits leads to diseases. Mutation in the subunit of eIF2B is associated with an autosomal recessive leukoencephalopathy called Vanishing white matter (VWM). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | eIF2Bε encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. |
| Immunogen: | synthetic peptide corresponding to amino acids 50-65 of human eIF2ε conjugated to KLH. The corresponding sequence is identical in rat and mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |