EZview™ Red Anti-c-Myc Affinity Gel
SIGMA/E6654
Synonym: Anti-c-Myc affinity resin; Anti-c-Myc agarose beads
Product Type: Chemical
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| analyte chemical class(es) | proteins (c-Myc) |
| capacity | ~0.05 mg/mL binding capacity |
| Quality Level | 200  |
| shelf life | 1 yr at 2‑8 °C |
| storage temp. | 2-8°C |
| technique(s) | affinity chromatography: suitable |
| immunoprecipitation (IP): suitable |
| Application: | EZview™ Red Anti-c-Myc Affinity Gel is suitable for: • SUMOylation assay • Affinity capture (immunoprecipitation) of N- or C-terminal c-Myc-tagged fusion proteins from E. coli or mammalian cells. |
| Application: | When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost. |
| Features and Benefits: | • Increased visibility - Red color reduces risk of incidental aspiration • Improved recovery of target protein by reduced accidental loss • Higher reproducibility - More consistent yields |
| General description: | EZ view Red Anti--c-Myc Affinity Gel is a resin that consists of the Anti-c-Myc rabbit antibody, covalently bonded to 4% Agarose beads. The affinity gel is used to bind c-Myc fusion proteins to samples, such as cell lysates and tissue, for purification of c-Myc-tagged proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results. Agarose beads bind at N-terminal, Met-N-terminal and C-terminal c-Myc fusion proteins from E. coli and mammalian cells. |
| Legal Information: | EZview is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | The affinity gel contains affinity purified anti-c-Myc rabbit polyclonal antibody covalently attached to beaded agarose. The c-Myc tag corresponds to amino acid residues 410-419 (EQKLISEEDL) of the human c-Myc protein. |
| Packaging: | 1, 5×1 mL in poly bottle |
| Physical form: | 1:1 (v/v) suspension in PBS containing 50% glycerol and 0.0015% Kathon CG/IPCII as an antimicrobial preservative |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |