Anti-eIF4ENIF1 antibody produced in rabbit
SIGMA/E6656 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-4E-T; Anti-Clast4; Anti-Eukaryotic translation initiation factor 4E nuclear import factor 1; Anti-Eukaryotic translation initiation factor 4E transporter; Anti-eIF4E-T
MDL Number: MFCD09753853
Product Type: Chemical
Immunoblotting Analysis of lysates from several cell lines, using eIF4ENIF1 antibody: The extracts were separated on SDA-PAGE and blotted with Rabbit Anti-eIF4ENIF1, Affinity isolated antibody (Cat. No. E6656), at a concentration of 2 μg/mL. The antibodies were developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminiscence substrate. Lysates from the following cell lines were loaded: Lane 1, HeLa; Lane 2, MCF7; Lane 3, A431; Lane 4, HepG2; Lane 5, Jurkat; Lane 6, K562
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 140 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μL using HeLa cell lysates |
| western blot: 2-4 μg/mL using HeLa cell lysates | |
| UniProt accession no. | Q9NRA8  |
| Application: | Anti-eIF4ENIF1 antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 5-10μL using HeLa cell lysates and western blotting at a concentration of 2-4μg/mL using HeLa cell lysates. |
| Application: | Anti-eIF4ENIF1 antibody produced in rabbit may be used in immunoblotting and immunoprecipitation. |
| Biochem/physiol Actions: | Anti-eIF4ENIF1 specifically recognizes human eIF4ENIF1. |
| Biochem/physiol Actions: | eIF4ENIF1 (also known as 4E-T or 4E-transporter) colocalizes with other mRNA binding/processing proteins to processing (P)-bodies. The interaction of eIF4ENIF1 with eIF4E in the cytoplasm represses translation, which is believed to be a prerequisite for targeting of mRNAs to P-bodies. eIF4ENIF1 is likely to inhibit translation by competing with eIF4G for binding to eIF4E and preventing the formation of the eIF4F complex. Together with 4E-BPs, it participates in cell response to prolonged hypoxia, sequestering eIF4E, and affecting gene expression. A nonsense mutation in the eIF4ENIF1 gene is implicated in the Primary Ovarian Insufficiency. |
| Biochem/physiol Actions: | Eukaryotic translation initiation factor 4E transporter (4E-T) is a protein encoded by the EIF4ENIF1 gene in humans. It is a nucleocytoplasmic shuttling protein that mediates the import of eIF4E into the nucleus. 4E-transporter (4E-T) is one of several proteins that binds the mRNA 5′cap-binding protein, eukaryotic initiation factor 4E (eIF4E), through a conserved binding motif. It′s interaction with eIF4E is a priming event in inducing messenger ribonucleoprotein rearrangement and transition from translation to decay. Eukaryotic initiation factor 4E plays an important role in controlling cell growth. 4E-T is essential for the assembly of processing bodies and its overexpression triggers the movement of eIF4E into the processing bodies. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Eukaryotic translation initiation factor 4E nuclear import factor 1 (eIF4ENIF1) is mapped to human chromosome 22q12.2. It expressed in the human ovary and comprises two leucine-rich nuclear export signals and a bipartite nuclear localization signal. |
| Immunogen: | synthetic peptide corresponding to amino acids 650-664 of human eIF4ENIF1, conjugated to KLH via an N-terminal added cysteine residue. This sequence is specific to human. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |