EZview™ Red Anti-HA Affinity Gel
SIGMA/E6779
Synonym: Anti-HA
Product Type: Chemical
| analyte chemical class(es) | proteins (hemagglutinin) |
| capacity | ≥0.4 mg/mL, gel binding capacity (HA-tagged fusion protein) |
| matrix | crosslinked agarose |
| Quality Level | 200  |
| shelf life | 1 yr at 2‑8 °C |
| storage temp. | 2-8°C |
| technique(s) | affinity chromatography: suitable |
| immunoprecipitation (IP): suitable |
| Application: | EZview™ Red Anti-HA Affinity Gel has been used for immunoblot analysis and immunoprecipitation assay. |
| Application: | Suitable for Affinity capture (immunoprecipitation) of N- or C- terminal HA-tagged fusion proteins from E. coli or mammalian cell. |
| Application: | When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduces the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost. |
| Biochem/physiol Actions: | Binding specificity: HA-tag peptide (N-Tyr-Pro-Tyr-Asp-Val-Pr |
| Features and Benefits: | • Increased visibility - Red color reduces risk of incidental aspiration • Improved recovery of target protein by reduced accidental loss • Higher reproducibility - More consistent yields |
| General description: | EZview Red Anti-HA Affinity Gel is a red colored Anti-HA agarose affinity gel that contains anti-HA monoclonal antibody which is covalently attached to crosslinked agarose beads. The HA tag has the sequence YPYDVPDYA and derives from the hemagglutinin (HA) protein. This sequence occurs at amino acid residues 98-106 of the full-length HA protein. The HA tag has a high presence of charged amino acids, which enhances its likelihood of strong antibody recognition. |
| Legal Information: | EZview is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | The affinity gel contains affinity purified anti-HA mouse monoclonal antibody (Clone HA-7) covalently attached to beaded agarose. The HA tag corresponds to amino acid residues 98-106 (YPYDVPDYA) of human influenza virus hemagglutinin (HA). |
| Physical form: | 1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |