pBICEP-CMV™-3 Expression Vector
SIGMA/E7029
Product Type: Chemical
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| Application: | For transient, cytoplasmic expression of an N-terminal FLAG® fusion and a second gene of interest or selection marker from bicistronic mRNA. Two genes can be cloned into MCS1 and MCS2 for transcription of a single message from the CMV promoter. This vector is useful for protein-protein interaction studies, multi-subunit proteins, and cloning a selection marker of choice. |
| Biochem/physiol Actions: | The presence of two multiple cloning sites not only allows the user to clone a target gene, but a selectable marker of their choice, as well. The promoter-regulatory region of the human cytomegalovirus immediate early promoter drives transcription of the FLAG-fusion construct along with a downstream selection gene. The EMCV IRES region controls translation of the selection gene by recruiting the ribosomal subunits for cap-independent translational initiation. Depending on the mode of selection, stable transfectants can be generated by transfection using the appropriate ESCORTä product line specific for the cell type. |
| General description: | The pBICEP-CMV-3 bicistronic expression vector is a 5.3 kb derivative of pCMV5 used for transient or stable co-expression of an N-terminal 1´ FLAGÒ fusion protein and a selection gene in mammalian cells. |
| Legal Information: | FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | pBICEP-CMV is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | Browse additional application references in our FLAG® Literature portal. |
| Packaging: | 20 μg in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12171500 |