EPHB1 (591-end), active, GST tagged from mouse
SIGMA/E7032 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: 9330129L11; AW488255; EPHT2; Elk; Elkh; Hek6; Net
Product Type: Chemical
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| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~62 kDa |
| product line | PRECISIO® Kinase |
| Quality Level | 200  |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 122-165 nmol/min·mg |
| storage temp. | −70°C |
| UniProt accession no. | Q8CBF3  |
| Application: | EPHB1 (591-end), active, GST tagged from mouse has been used for in vitro phosphorylation of Caskin1. |
| Biochem/physiol Actions: | EPHB1 (Ephb1 Eph receptor B1) is a member of the Eph family of receptor tyrosine kinases. Ligand-activated EPHB1 forms a signaling complex with Nck, paxillin, and focal adhesion kinase and induces tyrosine phosphorylation of paxillin in a c-Src-dependent manner to promote cell migration. In addition, activated EPHB1 recruits the adaptor proteins Grb2 and p52Shc and promotes p52Shc and c-Src tyrosine phosphorylation as well as MAPK/extracellular signal-regulated kinase (ERK) activation. Expression of dominant-negative c-Src significantly reduced EPHB1-dependent ERK1/2 activation and chemotaxis. This receptor is essential for the maintenance of hyperalgesia, and is the predominant receptor for chronic pain in the spinal cord. |
| General description: | EPHB1 (Ephb1 Eph receptor B1) is a glutamatergic synapse forming tyrosine kinase. Mammals contain five EPHB receptors, namely, EPHB1-EPHB4 and EPHB6. Their ligands are transmembrane in nature and are called ephrinB1, -B2 and -B3. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 122-165 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |