Earle’s Balanced Salt Solution 10x
SIGMA/E7510 - Without sodium bicarbonate, 10 ×, liquid, sterile-filtered, suitable for cell culture
Synonym: Earle’s Balanced Salts; EBSS; EBSS
MDL Number: MFCD00217343
Product Type: Chemical
| components | glucose: 10.0 g/L NaHCO3: no phenol red: 0.11 g/L |
| concentration | 10 × |
| form | liquid |
| impurities | endotoxin, tested |
| Quality Level | 400  |
| shipped in | ambient |
| sterility | sterile-filtered |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Earle′s Balanced Salt Solution 10x has been used: • as an additive to the nutritive medium sheep nematode Trichostrongylus colubriformis eggs • in the swim-up method to prepare human sperm sample • in the steady-state medium to culture bovine primary mammary epithelial cells |
| Features and Benefits: | • Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance. • Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. • When combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism. • Provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6). |
| General description: | Earle′s Balanced Salt Solution 10x is a saline solution with physiological pH. It is useful in inducing autophagy and promotes Bacillus Calmette-Guérin (BCG)-induced dendritic cells (DCs) maturation. |
| Preparation Note: | Supplement with 2.2 g/L sodium bicarbonate at 1×. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12352207 |