Anti-EEA1 antibody, Mouse monoclonal
SIGMA/E7659 - clone EEA1-N19, purified from hybridoma cell culture
Synonym: Anti-MST105; Anti-MSTP105; Anti-ZFYVE2; Monoclonal Anti-Early endosomal antigen 1
Product Type: Chemical
Immunoprecipitation
Monoclonal Anti-EEA1 Clone: EEA1-N19 (Cat. No. E7659) was used to immunoprecipitate EEA1 from HeLa (lane 2) and 3T3 (lanes 3-4) whole cell extracts. Detection antibody: Rabbit Anti-EEA1 (Cat. No. E4156).
Lanes
1. Positive control: 3T3 whole cell extract
2. 5 μg antibody
3. 5 μg antibody
4. Negative control: 5 μg non-relevant antibody
Monoclonal Anti-EEA1 Clone: EEA1-N19 (Cat. No. E7659) was used to immunoprecipitate EEA1 from HeLa (lane 2) and 3T3 (lanes 3-4) whole cell extracts. Detection antibody: Rabbit Anti-EEA1 (Cat. No. E4156).
Lanes
1. Positive control: 3T3 whole cell extract
2. 5 μg antibody
3. 5 μg antibody
4. Negative control: 5 μg non-relevant antibody
Immunoblotting Whole extract of NRK cells was separated on SDS-PAGE and probed with Monoclonal Anti-EEA1 Clone: EEA1-N19 (Cat. No. E7659). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 2 μg/mL antibody 2. 1 μg/mL antibody 3. Negative control: without 1st antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | EEA1-N19, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen ~160 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect immunofluorescence: suitable | |
| western blot: 1-2 μg/mL using whole extract of rat NRK cells | |
| UniProt accession no. | Q15075  |
| Application: | Anti-EEA1 antibody, Mouse monoclonalhas been used in: • immunoblotting • immunoprecipitation • cell fractionation • indirect immunofluorescence • immunohistochemistry |
| Application: | Monoclonal Anti-EEA1 antibody produced in mouse is suitable for the following applications: • Immunoprecipitation • Indirect immunofluorescence • Western blotting at a concentration of 1-2μg/mL using whole extract of rat NRK cells |
| Biochem/physiol Actions: | Early Endosome Antigen 1 (EEA1) controls vesicle fusion during endocytosis. In neurons, it is involved in recycling of synaptic vesicles and neurotransmitter receptors. Patients with neurological deficits develop EEA1 autoantibodies. Mutation in EEA1 gene leads to susceptibility to diabetes in the Japanese population. Phosphatidylinositol 3-phosphate (PtdIns(3)P) is essential for the localization and function of EEA1. |
| Biochem/physiol Actions: | Early Endosome Antigen 1 (EEA1) FYVE domain is involved in intracellular trafficking and is implicated in the specific localization of EEA1 to endosomes . Endosomal targeting of EEA1 also requires its binding to the active form of the small GTPase Rabaptin-5 (Rab5). The binding of EEA1 to phosphatidylinositol 3-phosphate (PtdIns 3P) and Rab5-GTP is essential for the localization and function of EEA1 in endocytic membrane fusion. Monoclonal Anti-EEA1 may be used as an early endosome marker. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-EEA1 (mouse IgG2a isotype) is derived from the hybridoma EEA1-N19 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human EEA1. Early Endosome Antigen 1 (EEA1) is a dimer. It comprises extensive coiled-coil regions, and at its C-terminus contains a cysteine-rich zinc-finger-like domain named FYVE domain. |
| General description: | The gene Early Endosome Antigen 1 (EEA1) encodes for around 1400 amino acid proteins. It is a peripheral membrane protein associated with the cytoplasmic face of early endosomes. EEA1 is a autoantigen associated with subacute cutaneous systemic lupus erythematosus and is a coiled-coil protein localized to early endosomes and cytosol. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |