Elastase from porcine pancreas
SIGMA/E7885 - lyophilized powder, suitable for cell culture
Synonym: Elastase from hog pancreas; Pancreatopeptidase E
CAS Number: 39445-21-1
EC Number: 254-453-6
MDL Number: MFCD00130998
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: E7885-1MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: E7885-5MG
| biological source | Porcine |
| foreign activity | Trypsin ≤50 BAEE units/mg protein |
| form | lyophilized powder |
| mol wt | 25.9 kDa |
| packaging | pkg of 1 μmol |
| pH | 8 |
| Quality Level | 200  |
| shipped in | dry ice |
| specific activity | ≥4 units/mg protein |
| storage temp. | −20°C |
| suitability | suitable for |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Elastase from porcine pancreas is used for the following applications: • Cell culture techniques • ELISA techniques • Used in assay for elastase inhibitory activity |
| Biochem/physiol Actions: | Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin. |
| Biochem/physiol Actions: | Enzyme that degrades elastin, a matrix component of tissues involved in stretching processes. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. |
| General description: | Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of N-succinyl- |
| Packaging: | 1, 5 mg in glass bottle |
| Packaging: | 20 mg in poly bottle |
| Packaging: | Package size based on protein content |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H334 - H335 |
| Precautionary statements | P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥4 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.21.36 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |