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Ethylenediaminetetraacetic acid solution

SIGMA/E8008 - 0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture

Synonym: EDTA solution

CAS Number: 60-00-4
Empirical Formula (Hill Notation): C10H16N2O8
Molecular Weight: 292.24
MDL Number: MFCD00003541
Linear Formula: C10H16N2O8
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-E8008-100ML 100 mL
 $21.30
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concentration 0.02% in DPBS (0.5 mM)
form solution
impurities endotoxin, tested
InChI 1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)
InChI key KCXVZYZYPLLWCC-UHFFFAOYSA-N
pH 7.1-7.7
product line BioReagent
Quality Level 200 
shipped in wet ice
SMILES string OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
sterility sterile-filtered
storage temp. 2-8°C
technique(s) cell culture | mammalian: suitable
Application: Ethylenediaminetetraacetic acid solution has been used to remove confluent cell monolayers in swine testicular (ST-cells) culture. It has also been used to detach embryonic stem cells (ESCs) from matrigel and primary human fibroblasts.
General description: Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and a hexadentate ligand. It chelates with metal ions to form an octahedral complex especially with cations. Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, which prevents clotting of blood. It is implicated in platelet aggregation in pseudothrombocytopenia. EDTA chelates with calcium and is effective in solubilisation of the atheromatous plaques. Calcium disodium EDTA administration is effective in slowing down the progression of chronic kidney disease. It is a chelator used to remove calcium from cell washing and suspension media. EDTA reduces cell clumping.
Physical form: Solution in Dulbecco′s phosphate buffered saline without calcium and magnesium
RIDADR NONH for all modes of transport
WGK Germany nwg
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. 2-8°C
UNSPSC 12352204

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