Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid tetrasodium salt

SIGMA/E8145 - ≥97%

Synonym: EGTA tetrasodium; Egtazic acid tetrasodium; Ethylene-bis(oxyethylenenitrilo)tetraacetic acid tetrasodium

CAS Number: 13368-13-3
Empirical Formula (Hill Notation): C₁₄H₂₀N₂O₁₀Na₄
Molecular Weight: 468.28
MDL Number: MFCD00274420
Linear Formula: C₁₄H₂₀N₂O₁₀Na₄
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-E8145-10G	10 g	$93.10 1/EA	Add To Favorites
45-E8145-50G	50 g	$340.00 1/EA	Add To Favorites

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Properties
Descriptions
Safety Info.
Basic Data

application(s)	life science and biopharma
assay	≥97%
InChI	1S/C14H24N2O10.4Na/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24;;;;/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24);;;;/q;4*+1/p-4
InChI key	BAOGCDPNNOBWQZ-UHFFFAOYSA-J
Quality Level	200
SMILES string	[Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CCOCCOCCN(CC([O-])=O)CC([O-])=O)CC([O-])=O
solubility	H₂O: soluble 10%, clear, colorless
technique(s)	RNA purification: suitable

Application:	A chelating agent useful for the determination of calcium in the presence of magnesium.
Application:	A chelating agent useful for the determination of calcium in the presence of magnesium. Repeated washing with EGTA-buffer can experimentally deplete cultured cells of Ca²⁺.
Application:	EGTA is suitable for use: • in media for chelating Ca²⁺ to facilitate efficient incorporation of Sr²⁺ into the oocytes • as a component of phosphatase wash buffer used to wash magnetic beads during dephosphorylation and radiolabeling of RNA segments cross-linked to immunoprecipitated proteins • as a component of oocyte activation media
Biochem/physiol Actions:	Ethylene glycol-bis(ß-aminoethyl ether)-N,N,N,N-tetra acetic acid tetrasodium salt (EGTA) is a calcium chelator that binds to Ca²⁺ in the presence of Mg²⁺ at a ratio of 1:1.
Biochem/physiol Actions:	Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetra acetic acid tetrasodium salt (EGTA) dissociates mucin complex and is useful in immunohistochemical studies. It suppresses root growth and mitotic index in germinating wheat seeds. It is used as a decalifying agent in root canal biomechanical preparation and is effective compared to EDTA and cyclohexanediaminetetraacetic acid (CDTA).
Packaging:	10, 50 g in glass bottle

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥97%
UNSPSC	12352202

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