Anti-eRF1 antibody produced in rabbit
SIGMA/E8156 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Eukaryotic translation termination factor 1; Anti-RF1; Anti-Release factor 1; Anti-eTF1
Product Type: Chemical
Western Blotting Whole extracts of Rat-1 cells were separated on SDS-PAGE and probed with Anti-eRF1 antibody produced in Rabbit (Cat. No. E8156). The antibody was developed using 1:30,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 2 μg/mL antibody 2. 1 μg/mL antibody 3. 0.5 μg/mL antibody 4. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 50 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 1-2 μg using HeLa cell lysates. |
| western blot: 0.5-1 μg/mL using 3T3 or Rat1 cell extracts. | |
| UniProt accession no. | P62495  |
| Application: | Anti-eRF1 antibody produced in rabbit has been used in western blotting and immunoprecipitation. |
| Biochem/physiol Actions: | Eukaryotic translation termination factor 1 (eRF1) proteins are directly implicated in the termination of translation in eukaryotes. It plays an important role in the termination of protein synthesis, which is performed by three domains (N, M and C) of eRF1. Rearrangement in eRF1 leads to N-domain accommodation into the ribosomal A site. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Eukaryotic translation termination factor 1 (eRF1) is a protein encoded by the ETF1 gene in humans. It belongs to eRF1 family and the amino-acid sequence is highly conserved. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |