Anti-E2F3 antibody ,Mouse monoclonal
SIGMA/E8651 - clone PG30, purified from hybridoma cell culture
MDL Number: MFCD00283127
Product Type: Chemical
Western Blotting Whole extract of 293T cells transfected with E2F3 were separated on SDS-PAGE and probed with Anti-E2F3 antibody, Mouse monoclonal, Clone: PG30 (Cat. No. E8651). The antibody was developed using 1:1,000 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A1682). Lanes 1. 4 μg/mL antibody 2. 8 μg/mL antibody
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | PG30, monoclonal |
| concentration | ~2 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen 57 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable using denatured E2F3 |
| microarray: suitable | |
| western blot: 4-8 μg/mL using transfected 293T cells expressing E2F3 | |
| UniProt accession no. | O00716  |
| Application: | Monoclonal Anti-E2F3 antibody produced in mouse has been used for the following applications: • immunoprecipitation using denatured E2F3 • western blotting at a concentration of 4-8μg/mL using transfected 293T cells expressing E2F3 • electrophoretic mobility shift assay (EMSA). |
| Biochem/physiol Actions: | Transcription factor E2F3 is involved in the regulation of cell cycle. E2F3 helps in the regulation of the expression of a number of genes that are important in cell proliferation. It is a oncogene with strong proliferative potential and is involved in many processes. E2F3 plays an important role in the development of several types of cancer. Overexpression of E2F3 may be associated with hepatocellular carcinoma (HCC). It plays an important role in controlling cell cycle progression and proliferation. E2F3 is frequently expressed in pediatric Wilms′ tumors and may be associated with the same. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-E2F3 (mouse IgG2a isotype) is derived from the PG30 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant human E2F Transcription Factor 3 (E2F3) protein. Transcription factor E2F3 is a DNA-binding protein of ~57 kDa encoded by the E2F3 gene in humans. It belongs to E2F transcription factor family of proteins that bind to the sequence TTTCGCGC. |
| Immunogen: | recombinant human E2F3. |
| Physical form: | Solution in phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide. |
| Preparation Note: | Purified from culture supernatant of hybridoma cells grown in a bioreactor. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |