Anti-E6AP antibody, Mouse monoclonal
SIGMA/E8655 - clone E6AP-330, purified from hybridoma cell culture
Synonym: Anti-E2F-6
MDL Number: MFCD04034520
Product Type: Chemical
Immunofluorescence Neuro-2a cells were fixed and permeabilized with cold methanol followed by acetone. Fixed cells were stained with 20 μg/mL Mouse Anti-E6AP, Clone: E6AP-330 (Cat. No. E8655). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunofluorescence NT2 cells were fixed and permeabilized with cold methanol followed by acetone. Fixed cells were stained with 20 μg/mL Mouse Anti-E6AP, Clone: E6AP-330 (Cat. No. E8655). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Immunoblotting Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Monoclonal Anti-E6AP, Clone: E6AP-330 (Cat. No. E8655). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HEK-293T; 2. NT2; 3. NIH-3T3; 4. Neuro-2a; 5. PC12; 6. COS-7; 7. MDCK; 8. CHO; 9. SH-SY-5Y; 10. SK-N-SH; 11. MDA-MB-231; 12. AT3B1; 13. PC3; 14. DU-145; 15. RAT2
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | E6AP-330, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~100 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1-2 μg/mL using total cell extract from 293T cells | |
| UniProt accession no. | O75461  |
| Application: | Monoclonal Anti-E6AP antibody is suitable for use in western blot (1-2 μg/mL using total cell extract from 293T cells), immunocytochemistry, immunoblotting, immunoprecipitation, indirect ELISA and microarray. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | E6AP is an E3 ubiquitin ligase that is expressed by the UB3A gene. Inhibiton or alterations of the UB3A gene may cause a neurological disorder called the Angelman Syndrome. E6AP interacts with E1 and E2 enzymes to mediate ubiquitination of proteins marked for degradation. E6AP also binds with the E6 viral protein present in HPV-infected cells. Monoclonal Anti-E6AP antibody is a useful tool for the study of E6AP and its function in protein degradation. This antibody is specific for E6AP protein in rat, mouse, human and monkey. |
| Immunogen: | human full-length recombinant E6AP. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |